Billington T, Nayudu P R
J Membr Biol. 1975 Apr 23;21(1-2):49-64. doi: 10.1007/BF01941061.
Brush border membranes have been isolated from villus epithelial cells of the adult Swiss mouse duodenum. Preparations of these membranes are not contaminated by other organelles as judged from electron-micrographs of sectioned pellets of brush borders. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins from brush borders solubilized in Tris-sodium dodecyl sulfate buffer reveals a reproducible Coomassie Brilliant Blue pattern of 17 bands. By comparing the brush border protein band positions with those of standard proteins run concurrently on sodium dodecyl sulfate-polyacrylamide gel slabs it is estimated that the 17 brush border proteins and subunits have molecular weights ranging from over 250,000 to around 16,000. Periodate-fuchsin sulfite staining shows that the five more slowly migrating, high molecular weight proteins are glycoproteins. The two proteins of smallest molecular size react positively with Oil Red O but have very small amounts of lipophilic amino acid residues, which indicates that the lipid extractable from the gels in these areas is a contaminant and is not bound to the proteins.
已从成年瑞士小鼠十二指肠的绒毛上皮细胞中分离出刷状缘膜。从刷状缘切片沉淀的电子显微照片判断，这些膜的制备物未被其他细胞器污染。用十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳分析溶解在Tris - 十二烷基硫酸钠缓冲液中的刷状缘蛋白，可得到17条带的可重复考马斯亮蓝图谱。通过将刷状缘蛋白条带位置与同时在十二烷基硫酸钠 - 聚丙烯酰胺凝胶板上运行的标准蛋白条带位置进行比较，估计这17种刷状缘蛋白和亚基的分子量范围从超过250,000到约16,000。高碘酸 - 品红亚硫酸盐染色显示，迁移较慢的五种高分子量蛋白是糖蛋白。两种分子量最小的蛋白与油红O呈阳性反应，但亲脂性氨基酸残基含量极少，这表明从这些区域的凝胶中提取的脂质是污染物，未与蛋白结合。
