Nakamura Wataru, Honma Sato, Shirakawa Tetsuo, Honma Ken-ichi
Department of Physiology, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan.
Nat Neurosci. 2002 May;5(5):399-400. doi: 10.1038/nn843.
Spontaneous discharges of individual neurons in the suprachiasmatic nucleus (SCN) of Clock mutant mice were recorded for over 5 days in organotypic slice cultures and dispersed cell cultures using a multi-electrode dish. Circadian rhythms with periods of about 27 hours were detected in 77% of slice cultures and 15% of dispersed cell cultures derived from Clock/Clock homozygotes. These findings indicate that the Clock mutation lengthens the circadian period but does not abolish the circadian oscillation, and suggest an important role of intercellular communication in the expression of circadian rhythm in the SCN.
使用多电极培养皿，在器官型切片培养物和分散细胞培养物中，对Clock突变小鼠视交叉上核（SCN）中单个神经元的自发放电进行了超过5天的记录。在源自Clock/Clock纯合子的77%的切片培养物和15%的分散细胞培养物中检测到了周期约为27小时的昼夜节律。这些发现表明，Clock突变延长了昼夜节律周期，但并未消除昼夜节律振荡，并提示细胞间通讯在SCN昼夜节律表达中起重要作用。
