Honma Sato, Nakamura Wataru, Shirakawa Tetsuo, Honma Ken-ichi
Department of Physiology, Hokkaido University, Graduate School of Medicine, North-15, West-7, Kita-ku, Sapporo 060-8638, Japan.
Neurosci Lett. 2004 Apr 1;358(3):173-6. doi: 10.1016/j.neulet.2004.01.022.
The circadian periods of single cultured neurons of the hypothalamic suprachiasmatic nucleus (SCN) in rats were assessed by means of multi-electrode array dish. Although the mean circadian period was not different between the dispersed cell culture and organotypic slice culture, the periods distributed in a wide range from 20.0 to 30.9 h in the former whereas concentrated in a narrow range in the latter. The same difference was also detected within each culture dish. There is a significant correlation between the period length and variation of circadian rhythm, where the more the mean circadian period in a culture dish deviates from the overall mean, the larger the standard deviation of period in a dish becomes. Such a correlation was not observed in the organotypic slice culture. These findings indicate that the diversity of circadian periods in the individual SCN neurons depends on the maintenance of SCN structure and the circadian period, suggesting that not only cell-to-cell communication but also the intrinsic circadian period plays a significant role in synchronizing the constitutional oscillators in the SCN.
通过多电极阵列培养皿评估大鼠下丘脑视交叉上核（SCN）单个培养神经元的昼夜节律周期。虽然分散细胞培养和器官型切片培养之间的平均昼夜节律周期没有差异，但前者的周期分布在20.0至30.9小时的较宽范围内，而后者则集中在较窄范围内。在每个培养皿中也检测到了相同的差异。昼夜节律周期长度与昼夜节律变化之间存在显著相关性，即培养皿中的平均昼夜节律周期偏离总体平均值越多，培养皿中周期的标准差就越大。在器官型切片培养中未观察到这种相关性。这些发现表明，单个SCN神经元中昼夜节律周期的多样性取决于SCN结构的维持和昼夜节律周期，这表明不仅细胞间通讯，而且内在的昼夜节律周期在同步SCN中的组成振荡器方面都起着重要作用。
