Nakamura Tetsu, Ajiki Tetsuo, Murao Shinichi, Kamigaki Takashi, Maeda Sakan, Ku Yonson, Kuroda Yoshikazu
Department of Biomedical Informatics, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.
Int J Oncol. 2002 May;20(5):937-41.
The calcium-binding protein S100A4 has been characterized as a metastasis-inducing molecule, and regulates cell motility and invasiveness of cancer cells. In order to clarify the significance of the expression of S100A4 as a prognostic factor in gallbladder cancer, S100A4 expression in resected gallbladder cancers were examined using an immunohistochemical staining technique. The relationship between S100A4 expression and clinicopathological factors including prognosis were evaluated. Twenty-five of 60 cases (42%) demonstrated positive staining for S100A4. There was no statistically significant association between S100A4 and histological grade, T, N, M factor, presence of stone, or stage. Kaplan-Meier method showed the 5-year survival rate of the group staining positive for S100A4 (31.5%) to be statistically poorer than that of the group staining negative for S100A4 (78.2%). Also in T2 cases, the 5-year survival rate of the group staining positive for S100A4 (57.1%) was statistically poorer than that of the group staining negative for S100A4 (83.3%). On univariate analysis, positive staining for S100A4 was a significant prognostic factor, and the hazard ratio was 4.05. On multivariate analysis, positive staining for S100A4 is also a significant predictor of prognosis second to T factor. These results indicate that positive staining for S100A4 is useful in assessing the prognosis of patients with gallbladder cancer as well as TNM factors.
钙结合蛋白S100A4已被确定为一种转移诱导分子，并调节癌细胞的细胞运动性和侵袭性。为了阐明S100A4表达作为胆囊癌预后因素的意义，采用免疫组织化学染色技术检测了切除的胆囊癌中S100A4的表达。评估了S100A4表达与包括预后在内的临床病理因素之间的关系。60例中有25例（42%）S100A4染色呈阳性。S100A4与组织学分级、T、N、M因子、结石存在情况或分期之间无统计学显著关联。Kaplan-Meier法显示，S100A4染色阳性组的5年生存率（31.5%）在统计学上低于S100A4染色阴性组（78.2%）。同样在T2病例中，S100A4染色阳性组的5年生存率（57.1%）在统计学上低于S100A4染色阴性组（83.3%）。单因素分析显示，S100A4染色阳性是一个显著的预后因素，风险比为4.05。多因素分析显示，S100A4染色阳性也是仅次于T因子的重要预后预测指标。这些结果表明，S100A4染色阳性在评估胆囊癌患者的预后以及TNM因素方面是有用的。
