Lee Hwan Hee, Park Jeong Seok, Chae Suhn Kee, Maeng Pil Jae, Park Hee Moon
Department of Microbiology, College of Natural Sciences, Chungnam National University, Daejeon 305-764, South Korea.
FEMS Microbiol Lett. 2002 Mar 5;208(2):253-7. doi: 10.1111/j.1574-6968.2002.tb11090.x.
Growth at the restrictive temperature (42 degrees C) of Aspergillus nidulans B120, carrying the conditional-lethal mutation sod(VI)C1, was partially improved by the addition of 1.0 M sorbitol to the medium. The mutant grown at 42 degrees C, with osmotic stabilizer, showed abnormal hyphal morphology, a decrease in beta-1,3-glucan synthase activity as well as cell wall sugar content, but an increase in chitin synthase activity and N-acetyl-glucosamine content. The mutation also affected the secretion of extracellular protease. The temperature-dependent osmo-sensitive phenotype of a Saccharomyces cerevisiae alpha-COP mutation can be rescued by the A. nidulans sod(VI)C(+) gene. These results indicate that the sod(VI)C1 mutation affects proper processing of secretory proteins destined for the surface of cells or beyond.
携带条件致死突变sod(VI)C1的构巢曲霉B120在限制温度（42摄氏度）下生长时，向培养基中添加1.0 M山梨醇可使其生长得到部分改善。在42摄氏度下添加渗透稳定剂培养的突变体表现出异常的菌丝形态，β-1,3-葡聚糖合酶活性以及细胞壁糖含量降低，但几丁质合酶活性和N-乙酰葡糖胺含量增加。该突变还影响细胞外蛋白酶的分泌。酿酒酵母α-COP突变的温度依赖性渗透敏感表型可被构巢曲霉sod(VI)C(+)基因拯救。这些结果表明，sod(VI)C1突变影响了 destined for the surface of cells or beyond.分泌蛋白的正确加工。 （原文中“destined for the surface of cells or beyond”表述不完整，翻译可能存在一定局限性）
