Byrne Bernadette, Iwata So
Department of Biological Sciences, Imperial College, SW7 2AZ, London, UK.
Curr Opin Struct Biol. 2002 Apr;12(2):239-43. doi: 10.1016/s0959-440x(02)00316-0.
A wide range of membrane protein structures have been published during the past two years. These have included proteins from both eucaryotic and heterologously overexpressed sources. Whereas some of these proteins were crystallised using conventional techniques, others employed the new methods of lipidic cubic phase crystallisation and antibody fragment co-crystallisation. These and other new approaches to the expression and crystallisation of membrane proteins are accelerating structural studies of membrane protein complexes.
在过去两年里,已经发表了大量的膜蛋白结构。这些结构包括来自真核生物和异源过表达来源的蛋白质。其中一些蛋白质是使用传统技术结晶的,而另一些则采用了脂立方相结晶和抗体片段共结晶的新方法。这些以及其他用于膜蛋白表达和结晶的新方法正在加速膜蛋白复合物的结构研究。