Witzmann Frank A, Li Junyu
Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA.
Am J Physiol Gastrointest Liver Physiol. 2002 May;282(5):G735-41. doi: 10.1152/ajpgi.00510.2001.
Technologies for proteomics, e.g., studies examining the protein complement of the genome, have been in development for over 20 years. More recently, proteomics has become formalized by combining techniques for large-scale protein separation with very precise, high-fidelity approaches that analyze, identify, and characterize the separated proteins. These methods bring to reality the powerful scope of proteomics, enabling researchers to investigate cellular function at the protein level and thus representing one of proteomics' most fitting applications. In this review, we take a brief and concise look at some of the current, physiologically relevant technologies that comprise proteomics and report specific applications in which proteomics has provided valuable biological insight.
蛋白质组学技术，例如研究基因组蛋白质组的技术，已经发展了20多年。最近，蛋白质组学通过将大规模蛋白质分离技术与非常精确、高保真的方法相结合而得以正式确立，这些方法用于分析、鉴定和表征分离出的蛋白质。这些方法实现了蛋白质组学强大的研究范围，使研究人员能够在蛋白质水平上研究细胞功能，因此代表了蛋白质组学最恰当的应用之一。在本综述中，我们简要且精炼地审视了构成蛋白质组学的一些当前与生理相关的技术，并报告了蛋白质组学提供了有价值生物学见解的具体应用。
