Orp150的N端截短形式是抗增殖蘑菇双孢蘑菇凝集素的一种胞质配体，是核定位序列依赖性核蛋白导入所必需的。
An N-terminal truncated form of Orp150 is a cytoplasmic ligand for the anti-proliferative mushroom Agaricus bisporus lectin and is required for nuclear localization sequence-dependent nuclear protein import.
作者信息
Yu Lu-Gang, Andrews Nigel, Weldon Mike, Gerasimenko Oleg V, Campbell Barry J, Singh Ravinder, Grierson Ian, Petersen Ole H, Rhodes Jonathan M
机构信息
Department of Medicine, The Henry Wellcome Laboratory of Molecular and Cellular Gastroenterology, University of Liverpool, Liverpool L69, United Kingdom.
出版信息
J Biol Chem. 2002 Jul 5;277(27):24538-45. doi: 10.1074/jbc.M203550200. Epub 2002 Apr 17.
Nuclear localization sequence-dependent nuclear protein import is essential for maintaining cell function and can be selectively blocked in epithelial cells by mushroom (Agaricus bisporus) lectin. Here we report that a major intracellular ligand for this lectin is an N-terminally truncated form of oxygen-regulated protein 150 (Orp150), which lacks the endoplasmic reticulum translocation signal peptide of full-length Orp150. This cytoplasmic form of Orp150 expresses the lectin carbohydrate ligand (sialyl-2,3-galactosyl-beta1,3-N-acetylgalactosamine-alpha) and is shown to be essential for nuclear localization sequence-dependent nuclear protein import.
依赖于核定位序列的核蛋白导入对于维持细胞功能至关重要，并且蘑菇（双孢蘑菇）凝集素可在上皮细胞中选择性地阻断这种导入。在此我们报告，这种凝集素的一种主要细胞内配体是氧调节蛋白150（Orp150）的N端截短形式，它缺乏全长Orp150的内质网转运信号肽。这种细胞质形式的Orp150表达凝集素碳水化合物配体（唾液酸基-2,3-半乳糖基-β1,3-N-乙酰半乳糖胺-α），并且已证明其对于依赖于核定位序列的核蛋白导入至关重要。
Zotero 插件