中国仓鼠细胞中的放线菌酮抗性。III. 多核糖体无细胞蛋白质合成的特性
Cycloheximide resistance in Chinese hamster cells. III. Characterization of cell-free protein synthesis by polysomes.
作者信息
Pöche H, Junghahn I, Geissler E, Bielka H
出版信息
Mol Gen Genet. 1975;138(2):173-7.
PMID:1196299
Abstract
Two clones were selected for mass cultivation from 18 phenotypically stable CHM-resistant CHO clones. The polysomes isolated from these two clones were compared with CHO wildtype polysomes and rat liver polysomes in a cell-free protein synthesis system for their ability to incorporate amino acids. CHM had an inhibitory effect on the protein synthesis activity of CHO wildtype and rat liver polysomes, but had no effect on the polysomes obtained from either of the mutant CHO clones.
摘要
从18个表型稳定的抗氯霉素（CHM）的中国仓鼠卵巢（CHO）克隆中挑选出两个克隆进行大规模培养。在无细胞蛋白质合成系统中，将从这两个克隆中分离出的多核糖体与CHO野生型多核糖体和大鼠肝脏多核糖体进行比较，以研究它们掺入氨基酸的能力。氯霉素对CHO野生型和大鼠肝脏多核糖体的蛋白质合成活性有抑制作用，但对从任何一个突变的CHO克隆中获得的多核糖体均无影响。
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