Wieslander L
Mol Biol Rep. 1975 Oct;2(3):189-94. doi: 10.1007/BF00356987.
It has earlier been shown by in situ hybridization that the 5 S RNA genes are located in region 2A of chromosome II in Chironomus tentans [1]. In the present study the resolution in this chromosome region could be considerably improved by using the inversion 1 A/9C of chromosome II, which carries region 2A, not in the usual position close to one end of chromosome II, but in the middle of the chromosome. It was then revealed that electrophoretically isolated 5 S RNA hybridized in situ with two adjacent bands in the region 2A. It was also observed that in heterozygotes harbouring one normal chromosome II and one with the inversion 1 A/9C, there is a close physical contact between the bands containing the 5 S RNA genes and the nucleolar organizer region.
原位杂交已经表明，在摇蚊中，5S RNA基因位于II号染色体的2A区域[1]。在本研究中，通过使用II号染色体的1A/9C倒位，该染色体区域的分辨率得到了显著提高。1A/9C倒位携带2A区域，它不在II号染色体靠近一端的正常位置，而是位于染色体中部。结果显示，电泳分离的5S RNA与2A区域的两条相邻条带进行原位杂交。还观察到，在含有一条正常II号染色体和一条带有1A/9C倒位染色体的杂合子中，含有5S RNA基因的条带与核仁组织区之间存在紧密的物理接触。
