Bäumlein H, Wobus U
Chromosoma. 1976 Aug 17;57(2):199-204. doi: 10.1007/BF00292918.
5 S RNA of Chironomus thummi larvae was purified from total phenol extracted RNA by gel filtration and labelled to about 10(7) dpm/mug with carrier-free iodine-125. After hybridization in situ of 125I-5 S RNA and autoradiography only region B3c-e (containing two "normal" and two very faint bands) of chromosome II of salivary gland cells was highly labelled. In chromosomes of an animal showing pairing discontinuities a clearly "heterozygous" labelling of the 5 S RNA region was found. Region B3c-e shows no clearcut morphological signs of puffing or autoradiographically detectable 3H-uridine incorportion in spite of a continuous synthesis of 5 S RNA in salivary gland cells.
从经总酚提取的RNA中通过凝胶过滤纯化出摇蚊幼虫的5S RNA，并用无载体的碘-125将其标记至约10⁷ dpm/μg。125I-5S RNA原位杂交及放射自显影后，仅唾液腺细胞第二条染色体的B3c - e区域（包含两条“正常”带和两条非常淡的带）被高度标记。在显示配对间断的动物染色体中，发现5S RNA区域有明显的“杂合”标记。尽管唾液腺细胞中5S RNA持续合成，但B3c - e区域未显示出明确的胀泡形态学迹象或放射自显影可检测到的3H-尿苷掺入。
