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蛙骨骼肌中糖酵解酶的可逆结合及含肌动蛋白丝的大小变化。

Reversible binding of glycolytic enzymes and size change in the actin-containing filaments of the frog skeletal muscle.

作者信息

Fulgenzi G, Graciotti L, Corsi A, Granata A L

机构信息

Institute of Experimental Pathology, Faculty of Medicine, University of Ancona, Italy.

出版信息

J Muscle Res Cell Motil. 2001;22(5):391-7. doi: 10.1023/a:1014553718306.

DOI:10.1023/a:1014553718306
PMID:11964065
Abstract

Electron microscopy was used in order to study the change of the actin-containing filaments when the bound glycolytic enzymes were removed by a high ionic strength solution. The effectiveness of the extraction medium was checked by estimating the aldolase activity released. Evidence was provided that the larger diameter of the actin filaments in the I-bands in comparison with the A-bands can be accounted for by the bound glycolytic enzymes.

摘要

为了研究当通过高离子强度溶液去除结合的糖酵解酶时含肌动蛋白丝的变化,采用了电子显微镜技术。通过评估释放的醛缩酶活性来检查提取介质的有效性。有证据表明,I带中肌动蛋白丝的直径比A带中的大,这可以由结合的糖酵解酶来解释。

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Reversible binding of glycolytic enzymes and size change in the actin-containing filaments of the frog skeletal muscle.蛙骨骼肌中糖酵解酶的可逆结合及含肌动蛋白丝的大小变化。
J Muscle Res Cell Motil. 2001;22(5):391-7. doi: 10.1023/a:1014553718306.
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本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Coupling of creatine kinase to glycolytic enzymes at the sarcomeric I-band of skeletal muscle: a biochemical study in situ.骨骼肌肌节I带中肌酸激酶与糖酵解酶的偶联:原位生化研究
J Muscle Res Cell Motil. 2000;21(7):691-703. doi: 10.1023/a:1005623002979.
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Ximdisp--A visualization tool to aid structure determination from electron microscope images.Ximdisp——一种辅助从电子显微镜图像确定结构的可视化工具。
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SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related fields.SPIDER和WEB:三维电子显微镜及相关领域图像的处理与可视化
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MRC image processing programs.医学研究委员会图像处理程序。
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Effects of low-frequency stimulation on soluble and structure-bound activities of hexokinase and phosphofructokinase in rat fast-twitch muscle.低频刺激对大鼠快肌中己糖激酶和磷酸果糖激酶的可溶性及与结构结合活性的影响。
Biochim Biophys Acta. 1995 Sep 6;1251(2):154-60. doi: 10.1016/0167-4838(95)00084-8.
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Interaction of aldolase with actin-containing filaments. Structural studies.醛缩酶与含肌动蛋白丝的相互作用。结构研究。
Biochem J. 1980 Jan 15;186(1):99-104. doi: 10.1042/bj1860099.
10
Binding of aldolase to actin-containing filaments. Evidence of interaction with the regulatory proteins of skeletal muscle.醛缩酶与含肌动蛋白丝的结合。与骨骼肌调节蛋白相互作用的证据。
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