Corsi A, Granata A L, Vecchi C, Strabbioli R, Re L
Laboratory of General Pathology, University of Ancona, Italy.
J Cell Sci. 1988 Aug;90 ( Pt 4):569-75. doi: 10.1242/jcs.90.4.569.
The sartorius muscle of Rana esculenta was fixed and dehydrated by different methods and cross-sections of sarcomeres of different lengths were examined by electron microscopy. The area within the outlines of the thin filaments in negatives was measured using a high-resolution television system and a graphics tablet or by using a microdensitometer and computer processing the images with a density threshold above the background level. In all specimens the area of the thin filaments in the I-bands was found to be larger than in the S-zones of the A-bands where the thick and thin filaments interpenetrate. The difference was statistically significant and independent of the length of the sarcomere. The results are in agreement with previous observations in glycerol-extracted fibres. It is suggested that the change in size of the thin filaments might be accounted for by some interaction with the thick filaments.
用不同方法固定并脱水食用蛙的缝匠肌，通过电子显微镜检查不同长度肌节的横截面。使用高分辨率电视系统和图形输入板测量底片上细肌丝轮廓内的面积，或者使用微密度计并通过计算机处理背景水平以上密度阈值的图像。在所有标本中，发现I带中细肌丝的面积大于A带S区中粗细肌丝相互穿插处的细肌丝面积。差异具有统计学意义，且与肌节长度无关。结果与先前在甘油提取纤维中的观察结果一致。有人提出，细肌丝大小的变化可能是由于与粗肌丝的某种相互作用所致。
