Sasaki Takayuki, Kamimura Saori, Takai Hiroyuki, Watanabe Akihiko, Onitake Kazuo
Department of Biology, Faculty of Science, Yamagata University, Japan.
Zygote. 2002 Feb;10(1):1-9. doi: 10.1017/s0967199402002010.
Localisation of the acrosome reaction inducing activity in egg-jelly was examined in the newt, Cynops pyrrhogaster. The jelly has six layers: the J0, J1, J2, J3, J4 and st layers. Jelly was mechanically dissected and placed on a Millipore filter. When sperm were added from the outer surface side of the jelly, most of them exhibited the acrosome reaction after passing through the jelly. When egg-jelly was divided into four layers, strong activity for the induction of acrosome reaction was detected in the outer layers, J4+st. These findings suggest that the acrosome reaction is induced by a substance in the outer layers of the egg-jelly. Among jelly components separated by SDS-PAGE, a fraction of more than 500 kDa in molecular weight induced the acrosome reaction. Wheat germ agglutinin (WGA), Griffonia simplicifoliar agglutinin 1 (GS-1), Maclura pomifera agglutinin (MPA) and Arachis hypogaea agglutinin (PNA) inhibited the induction of the acrosome reaction by jelly extract, and WGA did so in a dose-dependent manner. Those lectins precipitated some molecules of over 500 kDa. These results suggest that the acrosome reaction is induced by the high molecular-weight components of egg-jelly in C. pyrrhogaster.
在日本火腹蝾螈（Cynops pyrrhogaster）中，研究了卵胶中诱导顶体反应活性的定位。卵胶有六层：J0、J1、J2、J3、J4和st层。将卵胶进行机械切割并放置在微孔滤膜上。当从卵胶的外表面一侧添加精子时，大多数精子在穿过卵胶后发生顶体反应。当将卵胶分为四层时，在外层J4 + st中检测到强烈的诱导顶体反应的活性。这些发现表明，顶体反应是由卵胶外层中的一种物质诱导的。在通过SDS-PAGE分离的卵胶成分中，分子量超过500 kDa的一部分诱导了顶体反应。麦胚凝集素（WGA）、简叶豆凝集素1（GS-1）、桑橙凝集素（MPA）和花生凝集素（PNA）抑制了卵胶提取物诱导的顶体反应，并且WGA呈剂量依赖性。这些凝集素沉淀了一些分子量超过500 kDa的分子。这些结果表明，日本火腹蝾螈的顶体反应是由卵胶中的高分子量成分诱导的。
