半乳糖凝集素3蛋白（Gal3p）和半乳糖激酶1蛋白（Gal1p）与转录抑制因子半乳糖抑制蛋白80（Gal80p）相互作用，形成化学计量比为1:1的复合物。
Gal3p and Gal1p interact with the transcriptional repressor Gal80p to form a complex of 1:1 stoichiometry.
作者信息
Timson David J, Ross Helen C, Reece Richard J
机构信息
School of Biological Sciences, The University of Manchester, 2.205 Stopford Building, Oxford Road, Manchester M13 9PT, UK.
出版信息
Biochem J. 2002 May 1;363(Pt 3):515-20. doi: 10.1042/0264-6021:3630515.
Abstract
The genes encoding the enzymes required for galactose metabolism in Saccharomyces cerevisiae are controlled at the level of transcription by a genetic switch consisting of three proteins: a transcriptional activator, Gal4p; a transcriptional repressor, Gal80p; and a ligand sensor, Gal3p. The switch is turned on in the presence of two small molecule ligands, galactose and ATP. Gal3p shows a high degree of sequence identity with Gal1p, the yeast galactokinase. We have mapped the interaction between Gal80p and Gal3p, which only occurs in the presence of both ligands, using protease protection experiments and have shown that this involves amino acid residue 331 of Gal80p. Gel-filtration experiments indicate that Gal3p, or the galactokinase Gal1p, interact directly with Gal80p to form a complex with 1:1 stoichiometry.
摘要
酿酒酵母中编码半乳糖代谢所需酶的基因在转录水平上由一个由三种蛋白质组成的基因开关控制
一种转录激活因子Gal4p；一种转录抑制因子Gal80p；以及一种配体传感器Gal3p。该开关在两种小分子配体半乳糖和ATP存在的情况下开启。Gal3p与酵母半乳糖激酶Gal1p具有高度的序列同一性。我们利用蛋白酶保护实验绘制了Gal80p和Gal3p之间的相互作用图谱，这种相互作用仅在两种配体都存在时发生，并且已经表明这涉及Gal80p的氨基酸残基331。凝胶过滤实验表明，Gal3p或半乳糖激酶Gal1p与Gal80p直接相互作用，以1:1的化学计量比形成复合物。
相似文献
1
Gal3p and Gal1p interact with the transcriptional repressor Gal80p to form a complex of 1:1 stoichiometry.半乳糖凝集素3蛋白（Gal3p）和半乳糖激酶1蛋白（Gal1p）与转录抑制因子半乳糖抑制蛋白80（Gal80p）相互作用，形成化学计量比为1:1的复合物。
Biochem J. 2002 May 1;363(Pt 3):515-20. doi: 10.1042/0264-6021:3630515.
2
The yeast galactose genetic switch is mediated by the formation of a Gal4p-Gal80p-Gal3p complex.酵母半乳糖遗传开关由Gal4p-Gal80p-Gal3p复合物的形成介导。
EMBO J. 1998 Jul 15;17(14):4086-91. doi: 10.1093/emboj/17.14.4086.
3
Galactose induction in yeast involves association of Gal80p with Gal1p or Gal3p.酵母中的半乳糖诱导涉及Gal80p与Gal1p或Gal3p的结合。
Mol Gen Genet. 1999 Apr;261(3):495-507. doi: 10.1007/s004380050993.
4
Activation of Gal4p by galactose-dependent interaction of galactokinase and Gal80p.通过半乳糖激酶与Gal80p的半乳糖依赖性相互作用激活Gal4p。
Science. 1996 Jun 14;272(5268):1662-5. doi: 10.1126/science.272.5268.1662.
5
Dynamics of Gal80p in the Gal80p-Gal3p complex differ significantly from the dynamics in the Gal80p-Gal1p complex: implications for the higher specificity of Gal3p.Gal80p-Gal3p复合物中Gal80p的动力学与Gal80p-Gal1p复合物中的动力学有显著差异：对Gal3p更高特异性的影响。
Mol Biosyst. 2014 Dec;10(12):3120-9. doi: 10.1039/c4mb00371c.
6
Molecular simulation and docking studies of Gal1p and Gal3p proteins in the presence and absence of ligands ATP and galactose: implication for transcriptional activation of GAL genes.在有和没有配体 ATP 和半乳糖的情况下 Gal1p 和 Gal3p 蛋白的分子模拟和对接研究：对 GAL 基因转录激活的影响。
J Comput Aided Mol Des. 2012 Jul;26(7):847-64. doi: 10.1007/s10822-012-9579-5. Epub 2012 May 26.
7
The Gal3p-Gal80p-Gal4p transcription switch of yeast: Gal3p destabilizes the Gal80p-Gal4p complex in response to galactose and ATP.酵母的Gal3p-Gal80p-Gal4p转录开关：Gal3p在半乳糖和ATP存在时使Gal80p-Gal4p复合物不稳定。
Mol Cell Biol. 1999 Nov;19(11):7828-40. doi: 10.1128/MCB.19.11.7828.
8
Galactose-dependent reversible interaction of Gal3p with Gal80p in the induction pathway of Gal4p-activated genes of Saccharomyces cerevisiae.在酿酒酵母Gal4p激活基因的诱导途径中，Gal3p与Gal80p之间存在半乳糖依赖性可逆相互作用。
Proc Natl Acad Sci U S A. 1997 Mar 4;94(5):1721-6. doi: 10.1073/pnas.94.5.1721.
9
Analysis of the galactose signal transduction pathway in Saccharomyces cerevisiae: interaction between Gal3p and Gal80p.酿酒酵母中半乳糖信号转导途径的分析：Gal3p与Gal80p之间的相互作用
Mol Cell Biol. 1996 May;16(5):2504-8. doi: 10.1128/MCB.16.5.2504.
10
Gene activation by interaction of an inhibitor with a cytoplasmic signaling protein.通过抑制剂与细胞质信号蛋白的相互作用实现基因激活。
Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8548-53. doi: 10.1073/pnas.142100099.
引用本文的文献
1
Sustainable β-carotene production by engineered S. cerevisiae using sucrose and agricultural by-products.利用蔗糖和农业副产品通过工程改造的酿酒酵母实现可持续的β-胡萝卜素生产。
Bioresour Bioprocess. 2025 Sep 13;12(1):96. doi: 10.1186/s40643-025-00936-y.
2
Metabolic Engineering of for Production of Canthaxanthin, Zeaxanthin, and Astaxanthin.用于生产角黄素、玉米黄质和虾青素的代谢工程。
J Fungi (Basel). 2024 Jun 18;10(6):433. doi: 10.3390/jof10060433.
3
Ecological inducers of the yeast filamentous growth pathway reveal environment-dependent roles for pathway components.酵母丝状生长途径的生态诱导因子揭示了途径成分的环境依赖性作用。
mSphere. 2023 Oct 24;8(5):e0028423. doi: 10.1128/msphere.00284-23. Epub 2023 Sep 21.
4
Computational analysis of GAL pathway pinpoints mechanisms underlying natural variation.GAL 途径的计算分析确定了自然变异的潜在机制。
PLoS Comput Biol. 2021 Sep 27;17(9):e1008691. doi: 10.1371/journal.pcbi.1008691. eCollection 2021 Sep.
5
Computational study on ratio-sensing in yeast galactose utilization pathway.酵母半乳糖利用途径中比率感应的计算研究。
PLoS Comput Biol. 2020 Dec 4;16(12):e1007960. doi: 10.1371/journal.pcbi.1007960. eCollection 2020 Dec.
6
Multi-component gene network design as a survival strategy in diverse environments.多组分基因网络设计作为在不同环境中的一种生存策略。
BMC Syst Biol. 2018 Sep 26;12(1):85. doi: 10.1186/s12918-018-0609-3.
7
Genetic and Epigenetic Strategies Potentiate Gal4 Activation to Enhance Fitness in Recently Diverged Yeast Species.遗传和表观遗传策略增强 Gal4 激活，提高近期分化酵母物种的适应性。
Curr Biol. 2017 Dec 4;27(23):3591-3602.e3. doi: 10.1016/j.cub.2017.10.035. Epub 2017 Nov 16.
8
Noise reduction facilitated by dosage compensation in gene networks.剂量补偿促进基因网络中的降噪。
Nat Commun. 2016 Oct 3;7:12959. doi: 10.1038/ncomms12959.
9
Ongoing resolution of duplicate gene functions shapes the diversification of a metabolic network.重复基因功能的持续分化塑造了代谢网络的多样性。
Elife. 2016 Sep 30;5:e19027. doi: 10.7554/eLife.19027.
10
A cell size- and cell cycle-aware stochastic model for predicting time-dynamic gene network activity in individual cells.一种用于预测单个细胞中时间动态基因网络活性的细胞大小和细胞周期感知随机模型。
BMC Syst Biol. 2015 Dec 9;9:91. doi: 10.1186/s12918-015-0240-5.
本文引用的文献
1
Gal80-Gal80 interaction on adjacent Gal4p binding sites is required for complete GAL gene repression.相邻Gal4p结合位点上的Gal80-Gal80相互作用是GAL基因完全抑制所必需的。
EMBO J. 2001 Feb 15;20(4):841-51. doi: 10.1093/emboj/20.4.841.
2
Evidence for Gal3p's cytoplasmic location and Gal80p's dual cytoplasmic-nuclear location implicates new mechanisms for controlling Gal4p activity in Saccharomyces cerevisiae.半乳糖凝集素3蛋白（Gal3p）定位于细胞质以及半乳糖抑制蛋白80（Gal80p）定位于细胞质和细胞核的证据，揭示了酿酒酵母中控制半乳糖激活蛋白4（Gal4p）活性的新机制。
Mol Cell Biol. 2000 Jul;20(14):5140-8. doi: 10.1128/MCB.20.14.5140-5148.2000.
3
The insertion of two amino acids into a transcriptional inducer converts it into a galactokinase.在转录诱导剂中插入两个氨基酸会将其转化为半乳糖激酶。
Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3154-9. doi: 10.1073/pnas.97.7.3154.
4
The Gal3p-Gal80p-Gal4p transcription switch of yeast: Gal3p destabilizes the Gal80p-Gal4p complex in response to galactose and ATP.酵母的Gal3p-Gal80p-Gal4p转录开关：Gal3p在半乳糖和ATP存在时使Gal80p-Gal4p复合物不稳定。
Mol Cell Biol. 1999 Nov;19(11):7828-40. doi: 10.1128/MCB.19.11.7828.
5
The yeast galactose genetic switch is mediated by the formation of a Gal4p-Gal80p-Gal3p complex.酵母半乳糖遗传开关由Gal4p-Gal80p-Gal3p复合物的形成介导。
EMBO J. 1998 Jul 15;17(14):4086-91. doi: 10.1093/emboj/17.14.4086.
6
Signaling activation and repression of RNA polymerase II transcription in yeast.酵母中RNA聚合酶II转录的信号激活与抑制
Bioessays. 1997 Nov;19(11):1001-10. doi: 10.1002/bies.950191110.
7
Novel Gal3 proteins showing altered Gal80p binding cause constitutive transcription of Gal4p-activated genes in Saccharomyces cerevisiae.显示Gal80p结合改变的新型Gal3蛋白导致酿酒酵母中Gal4p激活基因的组成型转录。
Mol Cell Biol. 1997 May;17(5):2566-75. doi: 10.1128/MCB.17.5.2566.
8
Galactose-dependent reversible interaction of Gal3p with Gal80p in the induction pathway of Gal4p-activated genes of Saccharomyces cerevisiae.在酿酒酵母Gal4p激活基因的诱导途径中，Gal3p与Gal80p之间存在半乳糖依赖性可逆相互作用。
Proc Natl Acad Sci U S A. 1997 Mar 4;94(5):1721-6. doi: 10.1073/pnas.94.5.1721.
9
Activation of Gal4p by galactose-dependent interaction of galactokinase and Gal80p.通过半乳糖激酶与Gal80p的半乳糖依赖性相互作用激活Gal4p。
Science. 1996 Jun 14;272(5268):1662-5. doi: 10.1126/science.272.5268.1662.
10
Analysis of the galactose signal transduction pathway in Saccharomyces cerevisiae: interaction between Gal3p and Gal80p.酿酒酵母中半乳糖信号转导途径的分析：Gal3p与Gal80p之间的相互作用
Mol Cell Biol. 1996 May;16(5):2504-8. doi: 10.1128/MCB.16.5.2504.
Zotero 插件