Borovanský J, Hach P, Duchon J
Neoplasma. 1975;22(5):525-30.
The procedure for isolating soluble melanoprotein from human malignant melanoma was described. Electron microscopy confirmed the absence of any contaminating organized particles. The melanoprotein was brown hygroscopic substance with 8.8% of melanin, easily soluble in water. The solution revealed general absorption with a shoulder at 280 nm. Chemical composition was typical for a melanoprotein complex with characteristic high sulphur content (2.1%) and abundance in zinc (210 mug Zn/g dry sample). Sedimentation and electrophoretic properties indicated relative homogeneity. Similar substance could also be isolated from Harding-Passey mouse melanoma. Such soluble melanoproteins have been needed for their broad exploitation in tumor immunology.
描述了从人类恶性黑色素瘤中分离可溶性黑素蛋白的方法。电子显微镜证实不存在任何污染的有组织颗粒。黑素蛋白是一种棕色吸湿物质，含有8.8%的黑色素，易溶于水。该溶液在280nm处有一个肩峰的一般吸收峰。其化学成分是典型的黑素蛋白复合物，具有特征性的高硫含量（2.1%）和丰富的锌含量（210微克锌/克干样品）。沉降和电泳性质表明其相对均一性。类似的物质也可以从小鼠哈丁-帕西黑色素瘤中分离出来。这种可溶性黑素蛋白因其在肿瘤免疫学中的广泛应用而被需要。
