García-Borrón J C, Saura M D, Solano F, Iborra J L, Lozano J A
Physiol Chem Phys Med NMR. 1985;17(2):211-8.
The alterations caused on the structure of melanins by acid and alkaline extraction methods were studied by means of techniques such as Fourier Transform Infrared spectroscopy and thermogravimetry. Acid extraction results in the hydrolysis of aromatic monomers of the pigment, leading to uncyclicized residues and to a less conjugated polymer. Alkaline methods catalyse the covalent binding of proteins present in the melanin extract, thus leading to melanoprotein artifacts. A new method based on the delipidation with ether and deproteinization with SDS of previously isolated melanosomes is proposed. This method shows that melanins present in Harding-Passey mouse melanoma have a bound protein moiety, so that they should exist "in vivo" as melanoprotein complexes. Thermogravimetric data suggest that the chromogen moiety of the melanoproteins in Harding-Passey mouse melanoma is a mixture of eu- and pheomelanins.
通过傅里叶变换红外光谱和热重分析等技术，研究了酸法和碱法提取方法对黑色素结构造成的改变。酸法提取会导致色素的芳香族单体发生水解，产生非环化残基和共轭程度较低的聚合物。碱法会催化黑色素提取物中蛋白质的共价结合，从而导致黑色素蛋白假象。提出了一种基于用乙醚脱脂和用十二烷基硫酸钠对先前分离的黑素小体进行脱蛋白的新方法。该方法表明，哈丁-帕西小鼠黑色素瘤中存在的黑色素具有结合蛋白部分，因此它们在“体内”应以黑色素蛋白复合物的形式存在。热重分析数据表明，哈丁-帕西小鼠黑色素瘤中黑色素蛋白的发色团部分是真黑素和褐黑素的混合物。
