Gäfvert E, Nilsson J L G, Hagelthorn G, Karlberg A-T
Department of Occupational Dermatology, National Institute for Working Life, SE-112 79 Stockholm, Sweden.
Br J Dermatol. 2002 Apr;146(4):649-56. doi: 10.1046/j.1365-2133.2002.04664.x.
For patients with allergic contact dermatitis, the main therapy is anti-inflammatory steroids, a non-specific and symptomatic treatment. In contact allergy, the antigen formation is considered to be the binding of a chemical (hapten) to a biological macromolecule, e.g. a protein. Limonene-2-hydroperoxide (Lim-OOH) is a hapten with a known allergenic effect. It is likely to bind to proteins in the skin via a radical mechanism. It might be possible to inhibit the allergic reaction by epidermal application of substances that can trap free radicals, e.g. antioxidants such as ascorbic acid or alpha-tocopherol, prior to the application of the hapten.
To study the influence of antioxidants on the allergenic effect of Lim-OOH in sensitization experiments on guinea pigs.
Pretreatment with the antioxidants was performed before induction to study the effect on sensitization as well as before challenge testing to study the effect on elicitation.
A reduction in the response rate was found both at sensitization and at elicitation. The antioxidants had no effect on cobalt allergy or on the allergenic effect of haptens that form antigens via nucleophilic-electrophilic reactions. No reduction of the effect was seen for irritants.
The protective effect of antioxidants in elicitation could be of practical therapeutic value, as it indicates a possibility for the treatment of patients who have become sensitized to haptens that form full antigens via a radical mechanism.
对于过敏性接触性皮炎患者，主要治疗方法是使用抗炎类固醇，这是一种非特异性的对症治疗。在接触性过敏中，抗原形成被认为是一种化学物质（半抗原）与生物大分子（如蛋白质）的结合。柠檬烯 - 2 - 氢过氧化物（Lim - OOH）是一种具有已知致敏作用的半抗原。它可能通过自由基机制与皮肤中的蛋白质结合。在应用半抗原之前，通过表皮涂抹能够捕获自由基的物质（如抗坏血酸或α - 生育酚等抗氧化剂），有可能抑制过敏反应。
在豚鼠致敏实验中研究抗氧化剂对Lim - OOH致敏作用的影响。
在诱导前进行抗氧化剂预处理以研究其对致敏的影响，在激发试验前进行预处理以研究其对激发的影响。
在致敏和激发阶段均发现反应率降低。抗氧化剂对钴过敏或通过亲核 - 亲电反应形成抗原的半抗原的致敏作用没有影响。对刺激物未观察到作用减弱。
抗氧化剂在激发阶段的保护作用可能具有实际治疗价值，因为这表明对于已对半抗原致敏且半抗原通过自由基机制形成完全抗原的患者有治疗的可能性。
