Hsieh Yao-Yuan, Chang Chi-Chen, Tsai Fuu-Jen, Hsu Yuan, Tsai Horng-Der, Tsai Chang-Hai
Department of Obstetrics and Gynecology, China Medical College Hospital, Taichung, Taiwan.
J Clin Lab Anal. 2002;16(3):121-6. doi: 10.1002/jcla.10021.
Interleukin-4 (IL-4) is a cytokine with anti-inflammatory properties. Tumor necrosis factor alpha (TNF-alpha), a pluripotent proinflammatory cytokine, plays an important role in the process of numerous inflammatory and autoimmune diseases. We aimed to investigate whether gene polymorphisms for the IL-4 -590 promoter, IL-4 intron3 and TNFalpha -308 promoter could be used as markers of susceptibility in endometriosis. The subjects, 226 premenopausal Taiwan Chinese women with surgically diagnosed endometriosis and nonendometriosis, were divided into two groups: 1) endometriosis (n = 120) and 2) nonendometriosis (n = 106). Polymorphisms for the IL-4 -590 promoter, IL-4 intron3, and TNFalpha -308 G-to-A promoter were detected by polymerase chain reaction (PCR). Genotypes and allelic frequencies for these gene polymorphisms in both groups were compared. We observed no significant differences in genotype distribution and allele frequency of the IL-4 -590 promoter, IL-4 intron3, and TNFalpha gene polymorphism between both groups. The proportions of the -590C homozygote/heterozygote/-590 T homozygote for the IL-4 promoter in both groups were 1.6/31.6/66.6% in group 1, and 5.8/33.0/61.2% in group 2. The proportions of the RP1 homozygote/heterozygote/RP2 homozygote for IL-4 intron3 in both groups were 62.5/34.1/2.5% (group 1), and 64.1/32.0/3.9% (group 2). The proportions of -308A homozygote/heterozygote/-308G homozygote for the TNFalpha promoter in both groups were 7.5/20.8/71.7% (group 1), and 7.5/17/75.5% (group 2). We concluded that there is no association between endometriosis and the IL-4 and TNFalpha gene polymorphisms. The IL-4 -590 promoter, IL-4 intron3, and TNFalpha -308 G-to-A polymorphisms are not useful markers for predicting susceptibility to endometriosis.
白细胞介素-4（IL-4）是一种具有抗炎特性的细胞因子。肿瘤坏死因子α（TNF-α）是一种多能促炎细胞因子，在众多炎症和自身免疫性疾病的过程中发挥重要作用。我们旨在研究IL-4 -590启动子、IL-4内含子3和TNFα -308启动子的基因多态性是否可作为子宫内膜异位症易感性的标志物。研究对象为226名经手术诊断为子宫内膜异位症和非子宫内膜异位症的绝经前台湾华裔女性，分为两组：1）子宫内膜异位症组（n = 120）和2）非子宫内膜异位症组（n = 106）。通过聚合酶链反应（PCR）检测IL-4 -590启动子、IL-4内含子3和TNFα -308 G→A启动子的多态性。比较两组中这些基因多态性的基因型和等位基因频率。我们观察到两组之间IL-4 -590启动子、IL-4内含子3和TNFα基因多态性的基因型分布和等位基因频率无显著差异。IL-4启动子-590C纯合子/杂合子/-590T纯合子在两组中的比例，第1组为1.6/31.6/66.6%，第2组为5.8/33.0/61.2%。IL-4内含子3的RP1纯合子/杂合子/RP2纯合子在两组中的比例，第1组为62.5/34.1/2.5%，第2组为64.1/32.0/3.9%。TNFα启动子-308A纯合子/杂合子/-308G纯合子在两组中的比例，第1组为7.5/20.8/71.7%，第2组为7.5/17/75.5%。我们得出结论，子宫内膜异位症与IL-4和TNFα基因多态性之间无关联。IL-4 -590启动子、IL-4内含子3和TNFα -308 G→A多态性不是预测子宫内膜异位症易感性的有用标志物。
