Pannicke Thomas, Bringmann Andreas, Reichenbach Andreas
Paul-Flechsig-Institut für Hirnforschung, Universität Leipzig, Germany.
Glia. 2002 May;38(3):268-72. doi: 10.1002/glia.10068.
The electrophysiology of murine Müller cells, and of their precursors, during postnatal development was investigated by using the whole-cell patch-clamp technique. Membrane potential, membrane capacitance, and expression of voltage-gated Na+ currents increased during the first 3 postnatal weeks. During the same period, the membrane resistance decreased due to an upregulation of both inward and outward K+ currents. Glutamate transporter-mediated currents increased during the first postnatal weeks, as well. In Müller (precursor) cells from rabbits, these transporter currents already achieved adult levels at postnatal day 6, the earliest stage studied. Together with the developmental time course of K+ currents, this indicates a delay in the maturation of murine Müller cells, as compared with cells from rabbit.
采用全细胞膜片钳技术研究了出生后发育过程中小鼠穆勒细胞及其前体细胞的电生理学特性。出生后的前三周，膜电位、膜电容和电压门控钠电流的表达均增加。在同一时期，由于内向和外向钾电流的上调，膜电阻降低。谷氨酸转运体介导的电流在出生后的头几周也增加。在兔的穆勒（前体）细胞中，这些转运体电流在出生后第6天（研究的最早阶段）就已达到成年水平。与钾电流的发育时间进程一起，这表明与兔细胞相比，小鼠穆勒细胞的成熟存在延迟。
