Frank-Kamenetskiĭ M D
Center for Advanced Biotechnology, Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA.
Mol Biol (Mosk). 2002 Mar-Apr;36(2):307-11.
Briefly discussed are experiments with single molecules, representing a novel trend in the biophysical study of DNA. The techniques of optical and magnetic tweezers whereby external force can be applied to individual DNA molecules were used to assess the structural transitions of the DNA double helix under such conditions. Discussed are the latest data on the dependence of the rate of complementary chain synthesis by DNA polymerase on the stretching of the template.
简要讨论了单分子实验，这代表了DNA生物物理研究中的一种新趋势。利用光学镊子和磁性镊子技术可以对单个DNA分子施加外力，以此来评估在这种条件下DNA双螺旋的结构转变。文中讨论了关于DNA聚合酶合成互补链的速率对模板拉伸的依赖性的最新数据。
