Spors Hartwig, Grinvald Amiram
The Department of Neurobiology, Weizmann Institute of Science, 76100, Rehovot, Israel.
Neuron. 2002 Apr 11;34(2):301-15. doi: 10.1016/s0896-6273(02)00644-x.
We explored the spatio-temporal dynamics of odor-evoked activity in the rat and mouse main olfactory bulb (MOB) using voltage-sensitive dye imaging (VSDI) with a new probe. The high temporal resolution of VSDI revealed odor-specific sequences of glomerular activation. Increasing odor concentrations reduced response latencies, increased response amplitudes, and recruited new glomerular units. However, the sequence of glomerular activation was maintained. Furthermore, we found distributed MOB activity locked to the nasal respiration cycle. The spatial distribution of its amplitude and phase was heterogeneous and changed by sensory input in an odor-specific manner. Our data show that in the mammalian olfactory bulb, odor identity and concentration are represented by spatio-temporal patterns, rather than spatial patterns alone.
我们使用一种新型探针，通过电压敏感染料成像（VSDI）技术，探究了大鼠和小鼠主嗅球（MOB）中气味诱发活动的时空动态。VSDI的高时间分辨率揭示了嗅小球激活的气味特异性序列。增加气味浓度可缩短反应潜伏期、增大反应幅度，并激活新的嗅小球单元。然而，嗅小球激活的序列保持不变。此外，我们发现主嗅球的分布式活动与鼻腔呼吸周期相关。其幅度和相位的空间分布是异质性的，并以气味特异性方式因感觉输入而改变。我们的数据表明，在哺乳动物的嗅球中，气味特征和浓度是由时空模式而非仅由空间模式来表征的。
