Burack W Richard, Cheng Alec M, Shaw Andrey S
Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA.
Curr Opin Immunol. 2002 Jun;14(3):312-6. doi: 10.1016/s0952-7915(02)00347-3.
Four non-enzymatic proteins form the structural core of the TCR signaling machinery, linking antigen-receptor activation to signaling. These four proteins, each with well defined protein-protein interaction domains, include three 'scaffolds' (LAT, SLP-76 and SLAP-130/Fyb/ADAP and a 'pure adaptor' (GADS). The biological functions of many distinct protein-protein interaction domains have been dissected through a methodological series of knockout and reconstitution experiments. In reviewing these recent advances, we attempt to address two questions often asked by immunologists not familiar with the field: what do scaffolds/adaptors/linkers do; and what do these terms mean?
四种非酶蛋白构成了TCR信号传导机制的结构核心，将抗原受体激活与信号传导联系起来。这四种蛋白，每种都有明确的蛋白质-蛋白质相互作用结构域，包括三种“支架蛋白”（LAT、SLP-76和SLAP-130/Fyb/ADAP）和一种“纯衔接蛋白”（GADS）。通过一系列方法性的敲除和重组实验，已经剖析了许多不同蛋白质-蛋白质相互作用结构域的生物学功能。在回顾这些最新进展时，我们试图回答免疫学家经常提出的两个问题：支架蛋白/衔接蛋白/连接蛋白的作用是什么；这些术语是什么意思？
