[自发性高血压大鼠血管平滑肌细胞增殖与血小板源性生长因子-AA及血小板源性生长因子受体-α表达的关系]

[Relationship between proliferation of vascular smooth muscle cells and PDGF-AA and PDGFR-alpha expression in SHRs].

作者信息

Jin Jun, Zhu Shan-Jun, Zhu Zhi-Ming, Yang Yong-Jian, Ding Gang

机构信息

Department of Cardiology, Xingqiao Hospital, Third Military Medical University, Chongqing 400037.

出版信息

Sheng Li Xue Bao. 2002 Apr 25;54(2):145-8.

PMID:11973595

Abstract

To explore the role of platelet derived growth factor-AA (PDGF-AA) and PDGFR-alpha expression in the proliferation and hypertrophy of vascular smooth muscle cells (VSMCs) in spontaneously hypertension rats (SHR), protein expression of PDGF-AA, PDGFR-alpha and PDGFR-beta in SHR/Wistar-Kyoto (WKY)-VSMC was observed by Western blot. Proliferation and hypertrophy of SHR-VSMCs induced by PDGF-AA were observed by measurement of PCNA and [(3)H] incorporation. PDGF-AA and PDGFR-alpha expression was markedly increased in SHR-VSMCs compared with that in WKY-VSMCs, but PDGFR-beta was not different in SHR and WKY-VSMCs. PDGF-AA induced PCNA expression and [(3)H] incorporation was increased in a dose-dependent manner in SHR, but not in WKY. It is suggested that an enhancement of PDGF-AA and PDGFR-alpha in SHRs may be one of the important factors for vascular modeling.

摘要

为探讨血小板衍生生长因子-AA（PDGF-AA）及血小板衍生生长因子受体-α（PDGFR-α）表达在自发性高血压大鼠（SHR）血管平滑肌细胞（VSMC）增殖和肥大中的作用，采用蛋白质免疫印迹法观察SHR/京都威斯塔大鼠（WKY）-VSMC中PDGF-AA、PDGFR-α和血小板衍生生长因子受体-β（PDGFR-β）的蛋白表达。通过检测增殖细胞核抗原（PCNA）和[³H]掺入量观察PDGF-AA诱导的SHR-VSMC增殖和肥大情况。与WKY-VSMC相比，SHR-VSMC中PDGF-AA和PDGFR-α表达明显增加，但SHR和WKY-VSMC中PDGFR-β无差异。PDGF-AA诱导的PCNA表达及[³H]掺入量在SHR中呈剂量依赖性增加，而在WKY中无此现象。提示SHR中PDGF-AA和PDGFR-α的增强可能是血管重塑的重要因素之一。