Siegfried B, Waser P G, Borebély A A, Huston J P
Physiol Behav. 1975 Jul;15(1):71-8. doi: 10.1016/0031-9384(75)90281-4.
A single wave of unilateral spreading depression in the cortex, hippocampus or caudate nucleus of rats elicits eating after 2-6 min. The present experiments provide evidence that such spreading depression-induced eating can be classically conditioned to a complex conditioned stimulus. A wave of spreading depression was triggered by injection of 0.5-2.0 mul of 25 percent KCl solution. In a first experiment successful conditioning was demonstrated in 20 rats, involving 7 cortical, 5 caudate and 8 hippocampal spreading depression sites. Four animals failed to show any conditioned eating. A control group of 8 animals, in which cortical spreading depression did not induce eating, showed no increase in eating in the presence of the CS after conditioning trials. A second experiment, which included pseudoconditioning and NaCl control groups, confirmed the results obtained in Experiment 1. Classical conditioning was successful in 11 animals, involving 6 cortical and 5 caudate spreading depression sites. Neither the pseudoconditioning (14 animals, involving 8 cortical and 6 caudate spreading depression sites) not the NaCl control group (8 animals, all with cortical sites), showed eating in the presence of the CS. In both experiments the conditioned eating underwent gradual extinction.
大鼠大脑皮层、海马体或尾状核的单次单侧扩散性抑制波在2至6分钟后会引发进食行为。目前的实验提供了证据表明，这种由扩散性抑制诱导的进食行为可以被经典条件反射到一个复杂的条件刺激上。通过注射0.5至2.0微升25%的氯化钾溶液引发扩散性抑制波。在第一个实验中，20只大鼠成功建立了条件反射，其中包括7个大脑皮层、5个尾状核和8个海马体扩散性抑制位点。4只动物未表现出任何条件性进食行为。一个由8只动物组成的对照组，其大脑皮层扩散性抑制不诱导进食，在条件反射试验后，在条件刺激出现时进食没有增加。第二个实验包括伪条件反射组和氯化钠对照组，证实了实验1的结果。11只动物成功建立了经典条件反射，其中包括6个大脑皮层和5个尾状核扩散性抑制位点。伪条件反射组（14只动物，包括8个大脑皮层和6个尾状核扩散性抑制位点）和氯化钠对照组（8只动物，均为大脑皮层位点）在条件刺激出现时均未表现出进食行为。在两个实验中，条件性进食行为都逐渐消退。
