Duchrow M, Windhövel U, Bethge T, Schwandner O, Markert U, Bruch H P, Broll R
Chirurgisches Forschungslabor, Klinik für Chirurgie, Universitätsklinikum Lübeck, Ratzeburger Allee 160, 23538 Lübeck.
Chirurg. 2002 Feb;73(2):154-8; discussion 158-60. doi: 10.1007/s00104-001-0375-3.
The aim of our study was to investigate the influence of polypropylene meshes on the proliferation and apoptosis of human cell cultures in vitro.
Human fibroblasts and HeLa cells were incubated in different densities (10(4), 3.10(4), 10(5) cells/well) together with polypropylene meshes (Prolene, 2 x 2 cm) in six-well culture dishes over a 48-h period. Cells without meshes served as controls. Cells were spun on slides and stained with the monoclonal antibody MIB-1. To calculate the proliferation indices, stained nuclei were counted. Apoptotic indices were determined by flow cytometric analysis, using FITC-conjugated Annexin-V and propidiumjodide for staining.
Fibroblasts showed only a slight reduction of the proliferation index (PI) from 64% (controls) to 60% (meshes). Increasing cell density leads to a decrease in the PI of both groups. The PI of HeLa cells was similar in mesh groups and controls and independent of cell density. The apoptotic index (AI) of fibroblasts was significantly higher in the mesh group (3.7%) in comparison with the controls (1.9%). The same was observed for HeLa cells (AI mesh group: 4.5%, AI controls: 1.2%). Furthermore, an increase of AI was found with increasing cell density in both cell lines.
Whereas meshes did not influence the proliferation of the cell lines examined, they seem to have a marked influence on apoptosis, as a significant increase of AI was observed in the mesh group in contrast to controls.
我们研究的目的是调查聚丙烯网片对体外培养的人细胞增殖和凋亡的影响。
将人成纤维细胞和HeLa细胞以不同密度（10⁴、3×10⁴、10⁵个细胞/孔）与聚丙烯网片（普理灵，2×2 cm）一起置于六孔培养板中培养48小时。无网片的细胞作为对照。将细胞离心涂片，用单克隆抗体MIB-1染色。为计算增殖指数，对染色的细胞核进行计数。通过流式细胞术分析确定凋亡指数，使用异硫氰酸荧光素（FITC）偶联的膜联蛋白V和碘化丙啶进行染色。
成纤维细胞的增殖指数（PI）仅略有降低，从64%（对照）降至60%（网片组）。细胞密度增加导致两组的PI均降低。HeLa细胞在网片组和对照组中的PI相似，且与细胞密度无关。成纤维细胞的凋亡指数（AI）在网片组（3.7%）显著高于对照组（1.9%）。HeLa细胞也观察到同样情况（网片组AI：4.5%，对照组AI：1.2%）。此外，在两种细胞系中均发现随着细胞密度增加AI升高。
虽然网片不影响所检测细胞系的增殖，但它们似乎对凋亡有显著影响，因为与对照组相比，网片组的AI显著增加。
