Weyhe Dirk, Belyaev Orlin, Buettner Goetz, Mros Kirsten, Mueller Christophe, Meurer Kirsten, Papapostolou Georgios, Uhl Waldemar
Department of Surgery, St Josef Hospital, Ruhr University, Bochum, Germany.
ANZ J Surg. 2008 Jan-Feb;78(1-2):55-60. doi: 10.1111/j.1445-2197.2007.04362.x.
Material amount and pore size have been recently discussed as probable important determinants of biocompatibility of mesh implants used in hernia repair. This study aimed to find out whether other constructional parameters affect the extent of early foreign body reaction in vitro.
An NRK-49F (mixed culture of normal rat kidney cells) fibroblast culture was incubated in the presence of a 'light' microporous mesh (35 g/m(2), 0.25 mm thick), a 'heavy' polypropylene knitted mesh (95 g/m(2), 0.55 mm thick) and a polypropylene/polyglactin composite mesh (35 g/m(2), 0.5 mm thick). A mesh-free cell suspension was used as a control group. Fibroblasts' proliferation, invasion and apoptosis rates were measured by commercially available quantification tests. Levels of tumour necrosis factor-alpha, transforming growth factor-beta1, interleukin (IL)-1 beta, IL-6 and IL-10 secreted by the fibroblasts in the supernatant were dynamically measured in a time kinetics of 6-96 h.
Invasion potential as well as proliferation and apoptosis rates of fibroblasts were enhanced by all meshes. The composite mesh stimulated the cell turnover with correspondingly increased levels of IL-6 and suppressed levels of transforming growth factor-beta1 significantly more than the two pure polypropylene meshes and the control group.
Early biological response of fibroblasts as a major component of foreign body reaction was most affected by the filament construction of the mesh combining polypropylene with multifilament, partially absorbable polyglactin fibres. Material reduction did not weaken foreign body reaction. Confirming previous findings from animal experiments and clinical observations, the described in vitro model seems to be an appropriate primary tool for studying the biological tolerance towards meshes.
材料数量和孔径最近被讨论为疝修补术中使用的网片植入物生物相容性的可能重要决定因素。本研究旨在确定其他结构参数是否会影响体外早期异物反应的程度。
将NRK - 49F（正常大鼠肾细胞混合培养物）成纤维细胞培养物与“轻质”微孔网片（35 g/m²，0.25 mm厚）、“重质”聚丙烯编织网片（95 g/m²，0.55 mm厚）和聚丙烯/聚乙交酯复合网片（35 g/m²，0.5 mm厚）一起孵育。无网片的细胞悬液用作对照组。通过市售的定量测试测量成纤维细胞的增殖、侵袭和凋亡率。动态测量上清液中由成纤维细胞分泌的肿瘤坏死因子-α、转化生长因子-β1、白细胞介素（IL）-1β、IL - 6和IL - 10在6 - 96小时时间动力学中的水平。
所有网片均增强了成纤维细胞的侵袭潜力以及增殖和凋亡率。与两种纯聚丙烯网片和对照组相比，复合网片对细胞更新的刺激作用相应地使IL - 6水平升高，并且显著抑制了转化生长因子-β1的水平。
作为异物反应主要成分的成纤维细胞的早期生物学反应受聚丙烯与复丝、部分可吸收的聚乙交酯纤维结合的网片的细丝结构影响最大。材料减少并未减弱异物反应。证实了先前动物实验和临床观察的结果，所述体外模型似乎是研究对网片生物耐受性的合适的初步工具。
