Frias-Lopez Jorge, Zerkle Aubrey L, Bonheyo George T, Fouke Bruce W
Department of Geology, University of Illinois, Urbana, Illinois 61801, USA.
Appl Environ Microbiol. 2002 May;68(5):2214-28. doi: 10.1128/AEM.68.5.2214-2228.2002.
Distinct partitioning has been observed in the composition and diversity of bacterial communities inhabiting the surface and overlying seawater of three coral species infected with black band disease (BBD) on the southern Caribbean island of Curaçao, Netherlands Antilles. PCR amplification and sequencing of bacterial 16S rRNA genes (rDNA) with universally conserved primers have identified over 524 unique bacterial sequences affiliated with 12 bacterial divisions. The molecular sequences exhibited less than 5% similarity in bacterial community composition between seawater and the healthy, black band diseased, and dead coral surfaces. The BBD bacterial mat rapidly migrates across and kills the coral tissue. Clone libraries constructed from the BBD mat were comprised of eight bacterial divisions and 13% unknowns. Several sequences representing bacteria previously found in other marine and terrestrial organisms (including humans) were isolated from the infected coral surfaces, including Clostridium spp., Arcobacter spp., Campylobacter spp., Cytophaga fermentans, Cytophaga columnaris, and Trichodesmium tenue.
在荷属安的列斯群岛库拉索岛南部，对三种感染了黑带病（BBD）的珊瑚物种的表面及其上覆海水所栖息的细菌群落的组成和多样性进行了观察，发现存在明显的分区。使用通用保守引物对细菌16S rRNA基因（rDNA）进行PCR扩增和测序，已鉴定出超过524个与12个细菌门类相关的独特细菌序列。分子序列显示，海水与健康、患黑带病以及死亡的珊瑚表面之间的细菌群落组成相似度低于5%。BBD细菌垫会迅速在珊瑚组织上迁移并杀死珊瑚组织。从BBD细菌垫构建的克隆文库由八个细菌门类和13%的未知序列组成。从受感染的珊瑚表面分离出了几个代表先前在其他海洋和陆地生物（包括人类）中发现的细菌的序列，包括梭菌属、弓形杆菌属、弯曲杆菌属、发酵噬纤维菌、柱状噬纤维菌和纤细束毛藻。
