Glöckner F O, Fuchs B M, Amann R
Max-Planck-Institut für Marine Mikrobiologie, Bremen, Germany.
Appl Environ Microbiol. 1999 Aug;65(8):3721-6. doi: 10.1128/AEM.65.8.3721-3726.1999.
Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes was used to investigate the phylogenetic composition of bacterioplankton communities in several freshwater and marine samples. An average of about 50% of the cells were detected by probes for the domains Bacteria and Archaea, and of these, about half could be identified at the subdomain level with a set of group-specific probes. Beta subclass proteobacteria constituted a dominant fraction in freshwater systems, accounting for 16% (range, 3 to 32%) of the cells, although they were essentially absent in the marine samples examined. Members of the Cytophaga-Flavobacterium cluster were the most abundant group detected in the marine systems, accounting for 18% (range, 2 to 72%) of the 4',6-diamidino-2-phenylindole (DAPI) counts, and they were also important in freshwater systems (7%, range 0 to 18%). Furthermore, members of the alpha and gamma subclasses of Proteobacteria as well as members of the Planctomycetales were detected in both freshwater and marine water in abundances <7%.
使用靶向rRNA的寡核苷酸探针进行荧光原位杂交（FISH），以研究几个淡水和海洋样本中浮游细菌群落的系统发育组成。通过针对细菌域和古菌域的探针平均检测到约50%的细胞，其中约一半可以用一组组特异性探针在亚域水平上进行鉴定。β-亚类变形菌在淡水系统中占主导部分，占细胞的16%（范围为3%至32%），不过在所检测的海洋样本中基本不存在。噬纤维菌-黄杆菌簇的成员是在海洋系统中检测到的最丰富的群体，占4',6-二脒基-2-苯基吲哚（DAPI）计数的18%（范围为2%至72%），它们在淡水系统中也很重要（7%，范围为0%至18%）。此外，在淡水和海水中均检测到变形菌α和γ亚类的成员以及浮霉菌门的成员，其丰度均<7%。
