酵母调节颗粒非ATP酶亚基6（Nas6p）的纯化、结晶及初步X射线衍射分析
Purification, crystallization and preliminary X-ray diffraction analysis of yeast regulatory particle non-ATPase subunit 6 (Nas6p).
作者信息
Adachi Naruhiko, Padmanabhan Balasundaram, Kataoka Kazuhiro, Kijima Kyoko, Yamaki Mariko, Horikoshi Masami
机构信息
Horikoshi Gene Selector Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Corporation (JST), 5-9-6 Tokodai, Tsukuba, Ibaraki 300-2635, Japan.
出版信息
Acta Crystallogr D Biol Crystallogr. 2002 May;58(Pt 5):859-60. doi: 10.1107/s0907444902004808. Epub 2002 Apr 26.
The regulatory particle non-ATPase subunit, Nas6p, from Saccharomyces cerevisiae has been crystallized by the hanging-drop vapour-diffusion method using PEG 4000 as precipitant. The crystals belong to the space group P2(1)2(1)2(1), with unit-cell parameters a = 41.43 (2), b = 61.74 (1), c = 98.09 (2) A, and contain one molecule in the asymmetric unit. A complete diffraction data set using synchrotron radiation was collected to 2.6 A resolution.
来自酿酒酵母的调节颗粒非ATP酶亚基Nas6p，已通过悬滴气相扩散法，以聚乙二醇4000作为沉淀剂进行了结晶。晶体属于空间群P2(1)2(1)2(1)，晶胞参数a = 41.43 (2)，b = 61.74 (1)，c = 98.09 (2) Å，不对称单元中含有一个分子。使用同步辐射收集了完整的衍射数据集，分辨率达到2.6 Å。
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