酿酒酵母硫氧还蛋白Trx1的表达、纯化、结晶及初步X射线衍射分析
Expression, purification, crystallization and preliminary X-ray diffraction analysis of thioredoxin Trx1 from Saccharomyces cerevisiae.
作者信息
Zhang Yaru, Bao Rui, Zhou Cong-Zhao, Chen Yuxing
机构信息
Institute of Protein Research, Tongji University, Shanghai 200092, People's Republic of China.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Apr 1;64(Pt 4):323-5. doi: 10.1107/S1744309108004612. Epub 2008 Mar 29.
Abstract
Thioredoxins play key roles in the cellular response to oxidative stress. Three isoforms of thioredoxin have been identified in Saccharomyces cerevisiae: two that are cytosolic (Trx1 and Trx2) and one that is mitochondrial (Trx3). In the present work, the cytosolic form Trx1 was cloned, expressed, purified and crystallized. Crystals were obtained by the hanging-drop vapour-diffusion method. A data set was collected from a single crystal to 1.7 A resolution. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 32.29, b = 46.59, c = 64.20 A, alpha = beta = gamma = 90 degrees .
摘要
硫氧还蛋白在细胞对氧化应激的反应中起关键作用。在酿酒酵母中已鉴定出三种硫氧还蛋白同工型：两种是胞质型（Trx1和Trx2），一种是线粒体型（Trx3）。在本研究中，克隆、表达、纯化并结晶了胞质型Trx1。通过悬滴气相扩散法获得晶体。从单晶收集了一套分辨率为1.7 Å的数据集。该晶体属于空间群P2(1)2(1)2(1)，晶胞参数a = 32.29、b = 46.59、c = 64.20 Å，α = β = γ = 90°。
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