El-Shami M, El-Kafafi S, Falconet D, Lerbs-Mache S
Laboratoire de Génétique Moléculaire des Plantes, UMR CNRS 5575-Université Joseph Fourier, CERMO, BP 53, 38041 Grenoble, France.
Mol Genet Genomics. 2002 Apr;267(2):254-61. doi: 10.1007/s00438-002-0660-y. Epub 2002 Mar 20.
In higher plants, the FtsZ protein, the ancestor of tubulin, has been shown to be implicated in both proplastid division, which occurs in dividing cells and in the division of the differentiated plastids present in non-dividing cells. Here we report studies on the expression of the two FtsZ gene families in higher plants, FtsZ1 and FtsZ2, in non-synchronized and synchronized tobacco BY2 cells. We have isolated and characterized members of each gene family from Nicotiana tabacum. Specific cDNA probes for each tobacco FtsZ gene family and polyclonal antibodies specific for the FtsZ1 and FtsZ2 proteins were obtained in order to determine mRNA and protein levels. A constant level of FtsZ1 and FtsZ2 transcripts and proteins was observed in non-synchronized cell cultures. However, a complex pattern of expression of both gene families was observed during the cell cycle in synchronized cells, with mRNA and protein levels peaking during cell division, thus implying that the FtsZ proteins may be involved in plastid transmission to the two daughter cells.
在高等植物中，微管蛋白的祖先FtsZ蛋白已被证明与质体前体分裂有关，质体前体分裂发生在分裂细胞中以及非分裂细胞中存在的分化质体的分裂过程中。在此，我们报告了在非同步化和同步化的烟草BY-2细胞中，高等植物中两个FtsZ基因家族（FtsZ1和FtsZ2）表达的研究。我们从烟草中分离并鉴定了每个基因家族的成员。为了确定mRNA和蛋白质水平，获得了针对每个烟草FtsZ基因家族的特异性cDNA探针以及针对FtsZ1和FtsZ2蛋白的多克隆抗体。在非同步化细胞培养物中观察到FtsZ1和FtsZ2转录本和蛋白质的水平恒定。然而，在同步化细胞的细胞周期中观察到两个基因家族复杂的表达模式，mRNA和蛋白质水平在细胞分裂期间达到峰值，因此表明FtsZ蛋白可能参与质体向两个子细胞的传递。
