人精浆中E和19-羟基E前列腺素的测定。
The measurement of E and 19-hydroxy E prostaglandins in human seminal plasma.
作者信息
Cooper I, Kelly R W
出版信息
Prostaglandins. 1975 Sep;10(3):507-14. doi: 10.1016/0090-6980(75)90131-8.
A method is described which measures the four main prostaglandins of human semen (PGE1, E2, 19-hydroxy PGE1, and 19-hydroxy PGE2). For routine measurements E1 and E2 are measured together as are 19-OH E1 and 19-OH E2. These are measured by forming oximes in aqueous solution extraction, methylation and trimethyl silylation followed by gas chromatography. The method has sufficient sensitivity to measure the levels found in the majority of semen samples. The normal range in men with proven fertility was 90 to 260 mug/ml of 19-hydroxy Es and 30-200 mug/ml of Es.
本文描述了一种测量人类精液中四种主要前列腺素（PGE1、E2、19-羟基PGE1和19-羟基PGE2）的方法。对于常规测量，E1和E2一起测量，19-OH E1和19-OH E2也一起测量。这些通过在水溶液中形成肟、萃取、甲基化和三甲基硅烷化，然后进行气相色谱法来测量。该方法具有足够的灵敏度来测量大多数精液样本中的含量。经证实有生育能力的男性的正常范围是19-羟基E为90至260微克/毫升，E为30至200微克/毫升。
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