Schöber Wolfgang, Wiskirchen Jakub, Kehlbach Rainer, Gebert Regina, Rodegerdts Enno, Betsch Angelika, Johst Ursula, Feuls Roland, Claussen Claus D, Duda Stephen D
Department of Diagnostic Radiology, Eberhard-Karls-Universität, Tübingen, Germany.
Invest Radiol. 2002 May;37(5):248-53. doi: 10.1097/00004424-200205000-00002.
The aim of the study was to examine the effects of azelastine on proliferation, clonogenic activity, cell-cycle, and migration of human aortic smooth-muscle cells (haSMCs) in vitro.
HaSMCs were treated for 4 days with azelastine (1 micromol/L, 25 micromol/L, 50 micromol/L). Half of the treated groups were incubated again with azelastine, the other half received azelastine-free medium every 4 days until day 20. The growth kinetics and clonogenic activity were assessed. The cell-cycle distribution was investigated by FACS -- analysis and the migratory ability was evaluated.
Azelastine inhibited the proliferation and the clonogenic activity of haSMCs in a dose dependent manner. A G2/M-phase block was induced and the migratory ability was significantly impaired.
Azelastine has the potential to inhibit the proliferation of haSMCs. If a sufficient dose can be applied either systemically or locally it could be a valuable substance to prevent restenosis.
本研究旨在检测氮卓斯汀对人主动脉平滑肌细胞（haSMCs）体外增殖、克隆形成活性、细胞周期及迁移的影响。
用氮卓斯汀（1微摩尔/升、25微摩尔/升、50微摩尔/升）处理haSMCs 4天。一半处理组每隔4天用氮卓斯汀再次孵育，另一半每4天更换为不含氮卓斯汀的培养基，直至第20天。评估生长动力学和克隆形成活性。通过流式细胞术分析研究细胞周期分布并评估迁移能力。
氮卓斯汀以剂量依赖方式抑制haSMCs的增殖和克隆形成活性。诱导了G2/M期阻滞，迁移能力显著受损。
氮卓斯汀有抑制haSMCs增殖的潜力。如果能全身或局部应用足够剂量，它可能是预防再狭窄的一种有价值的物质。
