Watanabe K, Nagasawa N, Wen F Q, Inoue K, Kuroda K, Yoshida M
Second Department of Medicine, School of Medicine, Fukuoka University, Japan.
Prostaglandins Leukot Essent Fatty Acids. 1997 Mar;56(3):209-11. doi: 10.1016/s0952-3278(97)90536-6.
We investigated the effects of azelastine, an anti-allergic agent, on the release of arachidonic acid metabolites from cultured human umbilical vein endothelial cells (HUVEC). High performance liquid chromatography (HPLC) revealed that HUVEC treated with 10(-5) M azelastine released smaller amounts of arachidonic acid metabolites into the medium than control HUVEC. Azelastine significantly reduced the release of prostaglandin F2 alpha (PGF2 alpha), leukotriene B4 (LTB4) and 5-hydroxy-eicosatetraenoic acid (5-HETE) by HUVEC. These results suggest that azelastine may inhibit contraction of bronchial smooth muscle cells and reduce bronchial inflammation by suppressing the release of arachidonic acid metabolites from vascular endothelial cells.
我们研究了抗过敏药氮卓斯汀对培养的人脐静脉内皮细胞（HUVEC）中花生四烯酸代谢产物释放的影响。高效液相色谱法（HPLC）显示，用10^(-5) M氮卓斯汀处理的HUVEC向培养基中释放的花生四烯酸代谢产物量比对照HUVEC少。氮卓斯汀显著减少了HUVEC释放前列腺素F2α（PGF2α）、白三烯B4（LTB4）和5-羟基二十碳四烯酸（5-HETE）。这些结果表明，氮卓斯汀可能通过抑制血管内皮细胞花生四烯酸代谢产物的释放来抑制支气管平滑肌细胞的收缩并减轻支气管炎症。
