大鼠脑提取物中6-甲氧基-1,2,3,4-四氢-β-咔啉的区域形成
Regional formation of 6-methoxy-1,2,3,4-tetrahydro-beta-carboline in rat brain extract.
作者信息
Hsu L L, Mandell A J
出版信息
Res Commun Chem Pathol Pharmacol. 1975 Oct;12(2):355-62.
In the presence of 5-methoxytryptamine (5-MeOT), 5-methyltetrahydrofolic acid (5-MTHF) yields 6-methoxy-1,2,3,4-tetrahydro-beta-carboline (6-MeOTHbetaC) in rat brain extracts, possibly via formaldehyde formation catalyzed by methylenetetrahydrofolate reductase. The formation of 6-MeOTHbetaC in selected brain regions, ranging from 452 +/- 40 pmol formed per mg protein per hour in corpus striatum to 119 +/- 17 pmol in cingulate cortex, is significantly correlated with the regional distribution of 1,2,3,4-tetrahydro-beta-carboline (THbetaC) formed from 5-MTHF and tryptamine (r = 0.76, p less than 0.01) as well as that of methylene-beta-phenylethylimine (MbetaphiEI) from 5-MTHF and beta-phenylethylamine (betaphiEA; r = 0.90, p less than 0.01). FAD enhances the activity, lowering both Vmax and Km values with respect to 5-MeOT and Vmax, but not Km, with respect to 5-MTHF.
在大鼠脑提取物中，5-甲氧基色胺（5-MeOT）存在的情况下，5-甲基四氢叶酸（5-MTHF）可能通过亚甲基四氢叶酸还原酶催化生成甲醛，进而产生6-甲氧基-1,2,3,4-四氢-β-咔啉（6-MeOTHβC）。在选定的脑区中，6-MeOTHβC的生成量从纹状体中每毫克蛋白质每小时生成452±40皮摩尔到扣带回皮质中119±17皮摩尔不等，与由5-MTHF和色胺生成的1,2,3,4-四氢-β-咔啉（THβC）的区域分布显著相关（r = 0.76，p < 0.01），也与由5-MTHF和β-苯乙胺（β-phiEA）生成的亚甲基-β-苯乙亚胺（MbetaphiEI）的区域分布显著相关（r = 0.90，p < 0.01）。黄素腺嘌呤二核苷酸（FAD）增强了该活性，降低了相对于5-MeOT的Vmax和Km值以及相对于5-MTHF的Vmax，但不降低Km值。
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