Song Zhenghua, Zhang Ni
Department of Chemistry, Northwest University, Xi'an 710069, China.
Bioorg Med Chem. 2002 Jul;10(7):2091-7. doi: 10.1016/s0968-0896(02)00086-x.
A selective and sensitive as well as rapid chemiluminescence (CL) flow sensor for the determination of analgin is described. The analytical reagents involved in chemiluminescence reaction, luminol and periodate, were both immobilized on an anion-exchange column. The CL signals produced by the reaction between luminol and periodate, which were eluted from the column through water injection, were decreased in the presence of analgin. Analgin was sensed by measuring the decrement of CL intensity, and which was observed linear over the logarithm of analgin concentration range of 0.1 to 50.0 ng mL(-1), and the limit of detection was 0.04 ng mL(-1) (3ó). At a flow rate of 2.0 mL min(-1), including sampling and washing, the detection could be performed in 0.5 min with a relative standard deviation of less than 3.0%. The proposed procedure was applied successfully in the monitoring of analgin in human urine samples without any pre-treatment process. It was found that the analgin concentration reached its maximum after being orally administrated for 4 h, and the analgin metabolism ratio in 10 h was 9.28% in the body of volunteers. The flow sensor offered reagentless procedures and remarkable stability in determination of analgin, and could be easily reused over 80 h.
本文描述了一种用于测定安乃近的选择性好、灵敏度高且快速的化学发光（CL）流动传感器。化学发光反应中涉及的分析试剂鲁米诺和高碘酸盐均固定在阴离子交换柱上。通过注水从柱中洗脱的鲁米诺和高碘酸盐之间反应产生的CL信号，在安乃近存在时会降低。通过测量CL强度的降低来检测安乃近，在0.1至50.0 ng mL⁻¹的安乃近浓度对数范围内观察到线性关系，检测限为0.04 ng mL⁻¹（3σ）。在流速为2.0 mL min⁻¹（包括进样和清洗）时，检测可在0.5分钟内完成，相对标准偏差小于3.0%。所提出的方法成功应用于未经任何预处理的人体尿液样本中安乃近的监测。结果发现，志愿者口服安乃近4小时后，安乃近浓度达到最大值，10小时内安乃近在体内的代谢率为9.28%。该流动传感器在安乃近测定中提供了无需试剂的方法和显著的稳定性，并且可以轻松重复使用超过80小时。
