Song Zhenghua, Hou Shuang
Department of Chemistry, Northwest University, Xi'an, 710069, Xi'an, People's Republic of China.
J Pharm Biomed Anal. 2002 May 15;28(3-4):683-91. doi: 10.1016/s0731-7085(01)00655-0.
A continuous flow sensor for the determination of thiamine was constructed by using controlled-reagent-release technology in a FIA-CL system. The analytical reagents, luminol and KIO(4), were both immobilized on an anion-exchange column. The CL signal produced by the reaction between luminol and KIO(4), which were eluted from the column through H(2)O injection, was decreased in the presence of thiamine. The decreased CL intensity was linear with thiamine concentration in the range 3.3 pmol ml(-1)-6.7 nmol ml(-1); and the limit of detection was 1.0 pmol ml(-1) (3.). The whole process, including sampling and washing, could be completed in 0.5 min with a relative standard deviation of less than 3.0%. The flow sensor showed remarkable stability and could be easily reused over 80 h. The sensor proposed was tested in determination of thiamine in pharmaceutical preparation and human urine samples.
采用流动注射化学发光系统中的控释试剂技术构建了一种用于测定硫胺素的连续流动传感器。分析试剂鲁米诺和高碘酸钾均固定在阴离子交换柱上。通过注入水从柱中洗脱出来的鲁米诺和高碘酸钾之间反应产生的化学发光信号，在硫胺素存在下会降低。化学发光强度的降低与硫胺素浓度在3.3皮摩尔/毫升 - 6.7纳摩尔/毫升范围内呈线性关系；检测限为1.0皮摩尔/毫升(3.)。整个过程，包括取样和清洗，可在0.5分钟内完成，相对标准偏差小于3.0%。该流动传感器显示出显著的稳定性，并且可以轻松重复使用超过80小时。所提出的传感器用于药物制剂和人尿液样品中硫胺素的测定。
