猫唾液腺在分泌过程中、缓激肽输注期间及慢性导管结扎后的跨毛细血管交换。

Transcapillary exchange in the cat salivary gland during secretion, bradykinin infusion and after chronic duct ligation.

作者信息

Mann G E, Smaje L H, Yudilevich D L

出版信息

J Physiol. 1979 Dec;297(0):355-67. doi: 10.1113/jphysiol.1979.sp013044.

DOI:10.1113/jphysiol.1979.sp013044

PMID:119844

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1458724/

Abstract

Capillary permeability-surface area products for 86Rb, [51Cr]EDTA (mol. wt. 357), [57Co]cyanocobalamin (mol. wt. 1353) and [125I]insulin (approximate mol. wt. 6000) have been measured using the single-circulation, multiple-tracer dilution technique in the in situ perfused submandibular salivary gland during parasympathetic nerve stimulation, close-arterial bradykinin infusion and following chronic duct ligation. 2. In glands with a natural blood supply, permeability-surface area for 86Rb and [51Cr]EDTA increased during parasympathetic stimulation, but this was shown to be related to the concomitant increase in blood flow rather than to a change in capillary permeability or in surface area. 3. In glands perfused at constant flow, parasympathetic stimulation led to a decrease in permeability-surface area for EDTA (-19.1 +/- 5.2%, mean +/- S.E., n = 5, P less than 0.05) cyanocobalamin (-12.3 +/- 6.0, n = 12, P less than 0.05), and insulin (-15.3 +/- 4.8, n = 11, P less than 0.02). It is suggested that this may be the result of a redistribution of flow from the acinar microcirculation to a less permeable ductal vasculature. 4. Bradykinin infusion had no significant effect on permeability-surface area for EDTA and cyanocobalamin in perfused glands. 5. In perfused glands, ligation of the submandibular duct for 3--12 days reduced permeability-surface area (ml.min-1.g-1) for [51Cr]EDTA from 5.26 +/- 0.60 (mean +/- S.E., n = 9) to 4.20 +/- 0.12 (n = 4, P less than 0.30), [57Co]cyanocobalamin from 3.22 +/- 0.12 (n = 48) to 2.02 +/- 0.08 (n = 15, P less than 0.001) and [125I]insulin from 1.52 +/- 0.07 (n = 39) to 0.72 +/- 0.23 (n = 11, P less than 0.001).

摘要

采用单循环、多示踪剂稀释技术，在副交感神经刺激、动脉内缓激肽输注期间以及慢性导管结扎后，对原位灌注的下颌下唾液腺中86Rb、[51Cr]乙二胺四乙酸（分子量357）、[57Co]氰钴胺素（分子量1353）和[125I]胰岛素（近似分子量6000）的毛细血管通透性-表面积乘积进行了测量。2. 在具有自然血液供应的腺体中，副交感神经刺激期间86Rb和[51Cr]乙二胺四乙酸的通透性-表面积增加，但这被证明与伴随的血流增加有关，而非毛细血管通透性或表面积的变化。3. 在以恒定流量灌注的腺体中，副交感神经刺激导致乙二胺四乙酸的通透性-表面积降低（-19.1±5.2%，平均值±标准误，n = 5，P<0.05），氰钴胺素降低（-12.3±6.0，n = 12，P<0.05），胰岛素降低（-15.3±4.8，n = 11，P<0.02）。提示这可能是血流从腺泡微循环重新分布至通透性较低的导管血管系统的结果。4. 缓激肽输注对灌注腺体中乙二胺四乙酸和氰钴胺素的通透性-表面积无显著影响。5. 在灌注腺体中，下颌下导管结扎3至12天，使[51Cr]乙二胺四乙酸的通透性-表面积（毫升·分钟-1·克-1）从5.26±0.60（平均值±标准误，n = 9）降至4.20±0.12（n = 4，P<0.30），[57Co]氰钴胺素从3.22±0.12（n = 48）降至2.02±0.08（n = 15，P<0.001），[125I]胰岛素从1.52±0.07（n = 39）降至0.72±0.23（n = 11，P<0.001）。