磷酸化染料木黄酮和磷酸黄豆苷元对大鼠股骨干骺端组织骨成分的体外合成代谢作用。

Anabolic effect of phosphogenistein and phosphodaidzein on bone components in rat femoral-metaphyseal tissues in vitro.

作者信息

Yamaguchi Masayoshi, Ma Zhong Jie, Fushimi Takashi

机构信息

Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan.

出版信息

J Bone Miner Metab. 2002;20(3):148-55. doi: 10.1007/s007740200021.

DOI:10.1007/s007740200021

PMID:11984697

Abstract

The effect of phosphogenistein and phosphodaidzein, which are phosphorylated for the hydroxyl group (OH) at the 7-position of genistein and daidzein, on bone components was investigated. Femoral-metaphyseal tissues obtained from male rats (4 weeks old) were cultured for 24-72 h in Dulbecco's modified Eagle's medium (high glucose, 4.5%) supplemented with antibiotics and bovine serum albumin. The presence of phosphogenistein (10(-5) M) caused a significant increase in calcium content, alkaline phosphatase activity, and deoxyribonucleic acid (DNA) content in bone tissues cultured for 24 h. Phosphodaidzein (10(-5) M) significantly elevated bone calcium and DNA content. These effects were completely prevented by the presence of cycloheximide (10(-6) M), an inhibitor of protein synthesis. When femoral-metaphyseal tissues were cultured for 48 h in the presence of parathyroid hormone (1-34) (PTH; 10(-8) M) or prostaglandin E2 (PGE2; 10(-6) M), bone calcium content was significantly decreased. This decrease was significantly blocked by the presence of phosphogenistein (10(-6) and 10(-5) M) or phosphodaidzein (10(-6) and 10(-5) M). The presence of PTH (10(-8) M) or PGE2 (10(-6) M) caused a significant increase in glucose consumption and lactic acid production by bone tissues. These increases were significantly inhibited by the presence of phosphogenistein (10(-5) M) or phosphodaidzein (10(-5) M), indicating their inhibitory effect on bone resorption. The present study has demonstrated that both phosphogenistein and phosphodaidzein have an anabolic effect on bone metabolism in rat femoral-metaphyseal tissues in vitro.

摘要

研究了染料木黄酮和黄豆苷元在7位羟基（OH）处磷酸化形成的磷酸化染料木黄酮和磷酸化黄豆苷元对骨成分的影响。从4周龄雄性大鼠获取股骨干骺端组织，在添加抗生素和牛血清白蛋白的杜尔贝科改良伊格尔培养基（高糖，4.5%）中培养24 - 72小时。磷酸化染料木黄酮（10⁻⁵ M）的存在导致培养24小时的骨组织中钙含量、碱性磷酸酶活性和脱氧核糖核酸（DNA）含量显著增加。磷酸化黄豆苷元（10⁻⁵ M）显著提高了骨钙和DNA含量。蛋白质合成抑制剂环己酰亚胺（10⁻⁶ M）的存在完全阻止了这些作用。当股骨干骺端组织在甲状旁腺激素（1 - 34）（PTH；10⁻⁸ M）或前列腺素E2（PGE2；10⁻⁶ M）存在下培养48小时时，骨钙含量显著降低。磷酸化染料木黄酮（10⁻⁶和10⁻⁵ M）或磷酸化黄豆苷元（10⁻⁶和10⁻⁵ M）的存在显著阻止了这种降低。PTH（10⁻⁸ M）或PGE2（10⁻⁶ M）的存在导致骨组织葡萄糖消耗和乳酸产生显著增加。磷酸化染料木黄酮（10⁻⁵ M）或磷酸化黄豆苷元（10⁻⁵ M）的存在显著抑制了这些增加，表明它们对骨吸收有抑制作用。本研究表明，磷酸化染料木黄酮和磷酸化黄豆苷元在体外对大鼠股骨干骺端组织的骨代谢均有合成代谢作用。