Dmoszyńska A, Roliński J, Bojarska-Junak A, Mańko J, Jawniak D, Walter-Croneck A, Soroka-Wojtaszko M, Hus M
Haematooncology Department, University School of Medicine, Lublin, Poland.
Pol J Pharmacol. 2001 Nov-Dec;53(6):709-13.
Supernatants from short-term culture of peripheral blood and bone marrow mononuclear cells obtained from 22 multiple myeloma patients were used to measure the concentration of TNF-alpha, HGF, IL-6 and its soluble receptor (sIL-6R), VEGF and bFGF. Cells were cultured with or without thalidomide (THAL). We observed statistically significant decrease in TNF-alpha, HGF, IL-6, sIL-6R in supernatants from THAL cultures compared to cells cultured without THAL. Flow cytometry technique was applied to study the Bcl2 expression on CD 4, CD 8 and CD 138 positive cells. The statistically significant decrease in Bcl2 expression on myeloma cells (CD 138+) was observed both in PB and BM cultures. THAL could inhibit the plasma cell growth both by diminishing proangiogenic cytokines production and enhancing myeloma cell apoptosis.
从22例多发性骨髓瘤患者获取的外周血和骨髓单个核细胞短期培养的上清液，用于检测肿瘤坏死因子-α（TNF-α）、肝细胞生长因子（HGF）、白细胞介素-6（IL-6）及其可溶性受体（sIL-6R）、血管内皮生长因子（VEGF）和成纤维细胞生长因子（bFGF）的浓度。细胞在有或没有沙利度胺（THAL）的情况下进行培养。我们观察到，与未用THAL培养的细胞相比，THAL培养的上清液中TNF-α、HGF、IL-6、sIL-6R有统计学意义的下降。应用流式细胞术研究Bcl2在CD4、CD8和CD138阳性细胞上的表达。在PB和BM培养物中均观察到骨髓瘤细胞（CD138+）上Bcl2表达有统计学意义的下降。THAL可通过减少促血管生成细胞因子的产生和增强骨髓瘤细胞凋亡来抑制浆细胞生长。
