Ono M, Shiina Y, Sakuma K, Inoue H
Department of Cytology, Kyorin University School of Health Sciences, Hachioji, Tokyo, Japan.
Cytopathology. 2002 Feb;13(1):46-53. doi: 10.1046/j.1365-2303.2002.00364.x.
Endometrial cytology is important not only for observing cytological findings, but also for assessing histological architecture. Therefore, we attempted to produce a histological preparation from the sample using an automatic fixation apparatus (ACF 1000) which employs a membrane filter method. After observation of the cytological features, the cover slip was removed and a paraffin-embedded section was prepared. Producing a histological section from a cytological specimen prepared with the ACF 1000 apparatus was more useful and easier than with the techniques described to date. Peelability of the cell was avoided by using a silane-coated membrane filter. Observation of the same cell cluster as that observed in the cytological sample was also facilitated in the histological section by this technique.
子宫内膜细胞学不仅对于观察细胞学发现很重要，而且对于评估组织学结构也很重要。因此，我们尝试使用采用膜滤法的自动固定装置（ACF 1000）从样本中制作组织学标本。观察细胞学特征后，移除盖玻片并制备石蜡包埋切片。用ACF 1000装置制备的细胞学标本制作组织学切片比迄今所描述的技术更有用且更简便。通过使用硅烷包被的膜滤器避免了细胞的剥离。通过该技术在组织学切片中也便于观察与细胞学样本中观察到的相同细胞簇。
