Ono Midori, Shiina Yoshio, Sakuma Kanae
Department of Cytology, Kyorin University School of Health Sciences, Hachioji, Tokyo, Japan.
Diagn Cytopathol. 2002 Jul;27(1):15-21. doi: 10.1002/dc.10129.
The presence of papillary cell clusters is taken as a serious indication in the diagnosis of well-differentiated endometrial adenocarcinoma in Japan. However, papillary-like clusters have been observed in both normal and benign samples. Therefore, overdiagnosis may occur in the observation of cytopreparations. The present study attempts to prepare a histological sample from an Auto Cyto Fix (ACF) sample prepared by the automatic fixation apparatus (ACF1000) using a membrane filter method after cytological observation and to examine its usefulness. In an ACF sample, a papillary-like cluster was observed and the coverslip was then removed, the circumference of the membrane filter was cut off, and the target cell cluster was smeared, embedded, and sliced. In the cases in which a papillary-like cluster was observed, even if differential diagnosis of the derivation was difficult due to a resemblance between the respective morphological findings, it was easily made by histological observation of an ACF sample.
在日本，乳头状细胞簇的存在被视为高分化子宫内膜腺癌诊断中的一个重要指征。然而，在正常和良性样本中均观察到了乳头状样簇。因此，在观察细胞涂片时可能会出现过度诊断的情况。本研究试图在细胞学观察后，使用膜滤法从自动固定装置（ACF1000）制备的自动细胞固定（ACF）样本中制备组织学样本，并检验其效用。在一个ACF样本中，观察到了一个乳头状样簇，然后移除盖玻片，切下膜滤器的周边，将目标细胞簇涂片、包埋和切片。在观察到乳头状样簇的病例中，即使由于各自形态学表现相似而难以进行来源的鉴别诊断，但通过对ACF样本进行组织学观察，很容易做出诊断。
