采用T细胞受体γ链基因聚合酶链反应诊断皮肤T细胞淋巴瘤累及中枢神经系统
T cell receptor gamma-chain gene polymerase chain reaction to diagnose central nervous system involvement by cutaneous T cell lymphoma.
作者信息
Taylor Robert, Vergilio Jo-Anne, Shapiro Michael, Raizen David, Hunt Jennifer, McGrath Cindy, Rook Alain, Bagg Adam
机构信息
Department of Neurology, Hospital of the University of Pennsylvania, 3400 Spruce Street, Philadelphia, PA 19104, USA.
出版信息
J Mol Diagn. 2002 May;4(2):118-20. doi: 10.1016/s1525-1578(10)60691-5.
The authors describe a patient who was suspected of having cutaneous T cell lymphoma involvement of the brain despite repeatedly negative cerebrospinal fluid (CSF) cytology, inconclusive flow cytometry, and no discrete lesion for brain biopsy. The diagnosis was made by polymerase chain reaction (PCR) analysis that showed a monoclonal T cell receptor gamma-chain gene rearrangement in the CSF, identically sized to that present in a skin biopsy specimen. Thus, PCR could be used early and routinely to diagnose central nervous system spread of T cell lymphomas, because of its potentially superior sensitivity and specificity to CSF cytology.
作者描述了一名患者，尽管脑脊液（CSF）细胞学检查多次呈阴性、流式细胞术结果不明确且无用于脑活检的离散病变，但仍怀疑其脑内有皮肤T细胞淋巴瘤浸润。通过聚合酶链反应（PCR）分析做出了诊断，该分析显示脑脊液中存在单克隆T细胞受体γ链基因重排，其大小与皮肤活检标本中的相同。因此，由于PCR对脑脊液细胞学检查可能具有更高的敏感性和特异性，它可早期且常规地用于诊断T细胞淋巴瘤的中枢神经系统扩散。
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