Shang Q, Zhang G, Xu C, Chen C X, Yu J G, Yan C Y
Institute of Liver Disease, Jinan Military Region, The 88th Hospital of PLA, Taian 271000,China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2001 Sep;15(3):277-9.
To investigate the expression of hepatitis B virus (HBV) antigen in brain tissue from liver cirrhosis patients with hepatitis B and explore its significance.
HBsAg and HBcAg were detected in the brain tissue from 70 liver cirrhosis dead patients with hepatitis B by S-P immunohistochemical assay, and the relationship between the expression of HBV antigen in brain tissue testing and clinic and pathology was analyzed.
30 patients (42.89%) were positive for HBV antigen. Among them, 24 patients (34.29%) were positive for HBsAg and 18 patients (25.71%) were positive for HBcAg. HBV antigen was mainly found in cytoplasm and distributed in neurons, neurogliocytes and vascular endothelial cells. The positive cells were distributed separately, scatteredly or focally. The expression of HBV antigen was not associated with the serum level of HBV replication but associated with the occurrence of hepatic encephalopathy (HE) and the severity of brain tissue pathologic lesions of HE.
The results indicate that HBV infection develop in brain tissue from liver cirrhosis patients with hepatitis B and HBV may replicate in it. HBV infection in brain tissue may p lay an important role in occurrence and development of HE from liver cirrhosis patients with hepatitis B.
探讨乙型肝炎肝硬化患者脑组织中乙肝病毒（HBV）抗原的表达情况并探究其意义。
采用S-P免疫组化法检测70例乙型肝炎肝硬化死亡患者脑组织中的HBsAg和HBcAg，并分析脑组织中HBV抗原表达与临床及病理的关系。
30例患者（42.89%）HBV抗原呈阳性。其中，24例患者（34.29%）HBsAg呈阳性，18例患者（25.71%）HBcAg呈阳性。HBV抗原主要位于细胞质中，分布于神经元、神经胶质细胞及血管内皮细胞。阳性细胞呈散在、局灶或片状分布。HBV抗原的表达与血清HBV复制水平无关，但与肝性脑病（HE）的发生及HE脑组织病理损害程度有关。
结果表明乙型肝炎肝硬化患者脑组织中存在HBV感染且可能在其中复制。脑组织中的HBV感染可能在乙型肝炎肝硬化患者HE的发生发展中起重要作用。
