Manak Michael S, Paul Anna-Lisa, Sehnke Paul C, Ferl Robert J
Plant Molecular and Cellular Biology, Horticulture Sciences Department, University of Florida, Gainesville, FL 32611-0690, USA.
Life Support Biosph Sci. 2002;8(2):83-91.
Transgenic arabidopsis plants containing the alcohol dehydrogenase (Adh) gene promoter fused to the green fluorescent protein (GFP) reporter gene were developed as biological sensors for monitoring physiological responses to unique environments. Plants were monitored in vivo during exposure to hypoxia, high salt, cold, and abcissic acid in experiments designed to characterize the utility and responses of the Adh/GFP biosensors. Plants in the presence of environmental stimuli that induced the Adh promoter responded by expressing GFP, which in turn generated a detectable fluorescent signal. The GFP signal degraded when the inducing stimulus was removed. Digital imaging of the Adh/GFP plants exposed to each of the exogenous stresses demonstrated that the stress-induced gene expression could be followed in real time. The experimental results established the feasibility of using a digital monitoring system for collecting gene expression data in real time from Transgenic Arabidopsis Gene Expression System (TAGES) biosensor plants during space exploration experiments.
开发了含有与绿色荧光蛋白（GFP）报告基因融合的乙醇脱氢酶（Adh）基因启动子的转基因拟南芥植物，作为监测对独特环境生理反应的生物传感器。在旨在表征Adh/GFP生物传感器的效用和反应的实验中，对暴露于缺氧、高盐、寒冷和脱落酸环境中的植物进行体内监测。存在诱导Adh启动子的环境刺激时，植物通过表达GFP做出反应，进而产生可检测的荧光信号。去除诱导刺激后，GFP信号降解。对暴露于每种外源胁迫下的Adh/GFP植物进行数字成像表明，胁迫诱导的基因表达可以实时跟踪。实验结果确立了在太空探索实验期间使用数字监测系统从转基因拟南芥基因表达系统（TAGES）生物传感器植物实时收集基因表达数据的可行性。
