Prajer M, Kościuszko H
Department of Experimental Zoology, Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Slawkowska 17, 31-016 Kraków, Poland.
Folia Biol (Krakow). 2001;49(3-4):125-8.
Approximately 1/2 of the macronucleoplasm of wild-type cells of Paramecium tetraurelia was transplanted into d4-84 mutant recipient homozygous for mutations nd3a (non-discharging trichocysts) and ts401 (temperature sensitive). After injection, 30% of surviving cells shifted from mutant to wild-type phenotype. Among the remaining cell lines 29% were unable to eject any trichocysts, and 41% discharged less than 10 of them per cell, when tested with picric acid. Observations were made through two successive vegetative fissions. These results showed that 30% of d4-84 cells contained foreign gene responsible for trichocyst discharge and produced cell lines of the wild-type phenotype, in which the expression as well as replication of this gene appeared normal.
将约二分之一的四膜虫野生型细胞的大核质移植到d4 - 84突变体受体中，该受体对nd3a（不释放刺丝泡）和ts401（温度敏感）突变纯合。注射后，30%存活的细胞从突变体表型转变为野生型表型。在用苦味酸测试时，在其余细胞系中，29%无法排出任何刺丝泡，41%每个细胞排出的刺丝泡少于10个。通过连续两次营养分裂进行观察。这些结果表明，30%的d4 - 84细胞含有负责刺丝泡释放的外源基因，并产生了野生型表型的细胞系，其中该基因的表达和复制似乎正常。
