Aufderheide K J
J Cell Sci. 1978 Jun;31:259-73. doi: 10.1242/jcs.31.1.259.
Wild-type and selected mutants of Paramecium tetraurelia were subjected to detailed analysis of the trichocyst phenotype. The characteristics of morphology, saltatory motility, and cortical insertion of trichocysts were found to be genetically distinguishable. In mutants with defective trichocyst saltatory motility, other intracellular motility systems (i.e. mitochondrial saltatory motility, and cyclosis) appeared to be unaffected. Saltatory motility appears to be an essential intermediate step in the functional development of the trichocyst, since it serves to transport the trichocyst from its site of assembly, deep in the cytoplasm, to the cortex, where it is inserted and becomes discharge-competent. If trichocyst saltatory motility is lost, as in the mutants ndA and tam8, then trichocysts are not transported to the cortex and are therefore not inserted. However, the successful transport of a trichocyst to the cortex does not guarantee the proper insertion of that trichocyst, as in the case of the mutant am. Apparently there are independent steps involved specifically with trichocyst insertion. The gross morphology of the trichocyst appears to be independent of its saltatory motility, and to a degree, independent of cortical insertion.
对四膜虫的野生型和选定突变体进行了刺丝泡表型的详细分析。发现刺丝泡的形态、跳跃运动及皮层插入特征在遗传上是可区分的。在刺丝泡跳跃运动有缺陷的突变体中，其他细胞内运动系统（即线粒体跳跃运动和胞质环流）似乎未受影响。跳跃运动似乎是刺丝泡功能发育中必不可少的中间步骤，因为它用于将刺丝泡从其在细胞质深处的组装位点运输到皮层，在那里它被插入并具备放电能力。如果像在ndA和tam8突变体中那样刺丝泡跳跃运动丧失，那么刺丝泡就不会被运输到皮层，因此也不会被插入。然而，如在am突变体的情况中，刺丝泡成功运输到皮层并不能保证该刺丝泡的正确插入。显然存在专门与刺丝泡插入相关的独立步骤。刺丝泡的总体形态似乎与其跳跃运动无关，并且在一定程度上与皮层插入无关。
