Noda Akihiro, Ohba Hiroyuki, Kakiuchi Takeharu, Futatsubashi Masami, Tsukada Hideo, Nishimura Shintaro
Advanced Technology Platform Research Laboratory, Fujisawa Pharmaceutical Co. Ltd., Ibaraki 300-2698, Japan.
Brain Res. 2002 May 17;936(1-2):76-81. doi: 10.1016/s0006-8993(02)02558-1.
Regional cerebral blood flow (rCBF) and regional cerebral metabolic rate of glucose (rCMRglc) were measured in aged and young monkeys by positron emission tomography (PET). Our purpose was to examine whether the age-related changes observed in the human brain also occur in the monkey brain. Studies were performed on six aged and six young-adult male rhesus monkeys (Macaca mulatta). rCBF and the rCMRglc were serially measured using PET with [(15)O]H(2)O and 2-[(18)F]fluoro-2-deoxy-D-glucose (FDG), respectively. In order to minimize the bias induced by anesthesia, the PET emission scans were performed in the conscious state. ROIs were taken for the cerebellum, hippocampus with adjacent cortex, striatum, occipital cortex, temporal cortex, frontal cortex and cingulate. Group differences and correlations between rCBF and rCMRglc in each group were determined. Aged monkeys had significantly lower rCBF in the cerebellum, hippocampus with the adjacent cortex, striatum, occipital cortex, temporal cortex, frontal cortex, and significantly lower rCMRglc in the cerebellum, hippocampus with the adjacent cortex, striatum, occipital cortex, temporal cortex, frontal cortex and cingulate, compared to young monkeys. There were significant correlations between rCBF and rCMRglc in both the aged and young groups, but no significant difference was found in relationship between the two groups. Age-related changes were observed not only in rCMRglc, but also in rCBF in aged monkeys, while the coupling between rCBF and rCMRglc was maintained even in aged monkeys. These results demonstrated the potential of aged monkeys to serve as an aged human model using PET.
通过正电子发射断层扫描（PET）测量了老年和幼年猴子的局部脑血流量（rCBF）和局部脑葡萄糖代谢率（rCMRglc）。我们的目的是研究在人类大脑中观察到的与年龄相关的变化是否也发生在猴子大脑中。对六只老年和六只年轻成年雄性恒河猴（猕猴）进行了研究。分别使用PET和[（15）O]H（2）O以及2-[（18）F]氟-2-脱氧-D-葡萄糖（FDG）连续测量rCBF和rCMRglc。为了尽量减少麻醉引起的偏差，在清醒状态下进行PET发射扫描。选取小脑、海马及其相邻皮质、纹状体、枕叶皮质、颞叶皮质、额叶皮质和扣带回作为感兴趣区（ROI）。确定了每组中rCBF和rCMRglc之间的组间差异和相关性。与幼年猴子相比，老年猴子的小脑、海马及其相邻皮质、纹状体、枕叶皮质、颞叶皮质、额叶皮质的rCBF显著降低，小脑、海马及其相邻皮质、纹状体、枕叶皮质、颞叶皮质、额叶皮质和扣带回的rCMRglc显著降低。老年组和年轻组的rCBF与rCMRglc之间均存在显著相关性，但两组之间的关系无显著差异。在老年猴子中，不仅观察到rCMRglc与年龄相关的变化，还观察到rCBF的变化，而即使在老年猴子中，rCBF与rCMRglc之间的耦合也得以维持。这些结果证明了老年猴子作为使用PET的老年人类模型的潜力。
