Hasegawa T, Kikuiri T, Takeyama S, Yoshimura Y, Mitome M, Oguchi H, Shirakawa T
Department of Oral Functional Science, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.
Tissue Cell. 2002 Feb;34(1):44-51. doi: 10.1054/tice.2002.0223.
The receptor activator of NF-kappa B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG), are the important proteins involved in osteoclastogenesis. In this study, we investigated the expressions of RANKL and OPG in cultured human periodontal ligament cells derived from deciduous teeth (DPDL cells) and their roles in osteoclastogenesis. Northern blotting revealed that the OPG mRNA was down-regulated by application of 10(-8) M 1 alpha, 25(OH)2 vitamin D3 [1,25-(OH)2D3] and 10(-7) M dexamethasone (Dex). In contrast, RANKL mRNA was up-regulated by the same treatment. Western blotting demonstrated a decrease in OPG following application of 1, 25-(OH)2D3 and Dex. Tartrate-resistant acid phosphatase (TRAP)-positive multinuclear cells (MNCs) were induced when DPDL cells were co-cultured with mouse bone marrow cells in the presence of 1,25-(OH)2D3 and Dex. TRAP-positive MNCs increased significantly when the DPDL cells were co-cultured with bone marrow cells in the presence of anti-human OPG antibody together with 1, 25-(OH)2D3 and Dex. These results indicate that PDL cells derived from deciduous teeth synthesize both RANKL and OPG and could regulate the differentiation of osteoclasts.
核因子-κB受体活化因子配体（RANKL）及其诱饵受体骨保护素（OPG）是参与破骨细胞生成的重要蛋白质。在本研究中，我们调查了人乳牙来源的牙周膜细胞（DPDL细胞）中RANKL和OPG的表达及其在破骨细胞生成中的作用。Northern印迹分析显示，应用10^(-8) M 1α,25(OH)2维生素D3 [1,25-(OH)2D3]和10^(-7) M地塞米松（Dex）可使OPG mRNA下调。相反，相同处理可使RANKL mRNA上调。Western印迹分析表明，应用1,25-(OH)2D3和Dex后OPG减少。当DPDL细胞在存在1,25-(OH)2D3和Dex的情况下与小鼠骨髓细胞共培养时，可诱导抗酒石酸酸性磷酸酶（TRAP）阳性多核细胞（MNCs）。当DPDL细胞在存在抗人OPG抗体以及1,25-(OH)2D3和Dex的情况下与骨髓细胞共培养时，TRAP阳性MNCs显著增加。这些结果表明，乳牙来源的牙周膜细胞可合成RANKL和OPG，并可调节破骨细胞的分化。
